Effect of magnesium alloy scaffolds on osteoblast function

doi:10.3969/j.issn.2095-4344.2016.34.001

Abstract

Abstract:

BACKGROUND: On the basis of the previous studies, the magnesium alloy is processed into porous scaffolds.
OBJECTIVE: To investigate the effect of porous magnesium alloy scaffolds on osteoblast function.
METHODS: MC3T3-E1 osteoblastic cell lines were seeded on the porous magnesium alloy scaffold or porous pure magnesium scaffold, respectively. After 6, 12 and 24 hours of incubation, the adhesion ability of osteoblasts on the scaffold was observed by acridine crange staining. At 1, 3 and 5 days after incubation, the proliferation ability of osteoblasts on the scaffold was evaluated by MTT assay; after 21-day incubation, the mineralization of osteoblasts was investigated using calcein staining.
RESULTS AND CONCLUSION: The number of osteoblasts adherent to the porous magnesium alloy scaffold was significantly more than that adherent to the porous pure magnesium scaffold after 24-hour incubation (P < 0.05). And the proliferation assay showed that a significantly higher absorbance was observed on the porous magnesium alloy scaffold than that of the porous pure magnesium scaffold after 3 and 5 days of incubation (P < 0.05). Moreover, the number and area of mineralized nodules formed on the the porous magnesium alloy scaffold were greater than those on the porous pure magnesium scaffold after 21-day incubation (P < 0.05). These results show that the porous magnesium alloy scaffold with an excellent bioactivity can promote the adhesion, proliferation and mineralization abilities of osteoblasts.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Magnesium, Osteoblasts, Tissue Engineering

CLC Number:

R318

Wang Yong-ping, Liu Xiao-rong, Zhang Bing-chun, He Yao-hua. Effect of magnesium alloy scaffolds on osteoblast function[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(34): 5021-5026.

Figures/Tables 5

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