Nano decalcified bone matrix promotes bone fusion

doi:10.3969/j.issn.2095-4344.2013.21.009

Abstract

Abstract:

BACKGROUND: Nano decalcified bone matrix has a bigger surface area/volume ratio. Irregular nano-cannelure will appear on the surface when nanoparticles are agglomerated, thereby promoting osteoblasts to grow and excrete matrix on the surface.
OBJECTIVE: To assess the bone graft and fusion of nano decalcified bone matrix as a bone graft substitute.
METHODS: The completely decalcified bone matrix was produced by improved Urist method, and the nano decalcified bone matrix was produced by liquid nitrogen frozen ball mill equipment and MICROS super fine mill. Nano decalcified bone matrix, decalcified bone matrix and autogenous bone were, respectively, implanted into the bilateral lumbar laminae and transverse process of rabbits undergoing removal of cortical bone on the surface of the bilateral lumbar laminae and transverse process. Fusion level at 4, 8 and 12 weeks after operation was observed through iconography and histology.
RESULTS AND CONCLUSION: X-ray and CT manifestations: 12 weeks after operation, the lumbar lamina of nano decalcified bone matrix group gained favorable bony fusion, the laminar space disappeared completely, and the bone mineral density of new bone and fusion bed was uniformity. There was a small amount of new bone tissues on the implanted region of decalcified bone matrix group, and the new bone did not gain favorable bony fusion. Autologous bone graft gained good bony fusion. Histological results: 12 weeks after operation, show that nano decalcified bone matrix was replaced by new bone, forming a bony connection with the lumbar lamina. A great quantity of osteocytes could be seen in the new bone after nano decalcified bone matrix grafting similar to autologuos bone grafting. The groups of decalcified bone matrix and autologous bone also gained bony fusion. In the decalcified bone matrix group, lamellar bone and neoplastic bone were visible in the new bone. Nano decalcified bone matrix possesses a better capacity of osteogenic induction, which is an outstanding substitute for autogenous bone.

Key words: biomaterials, nanobimaterials, decalcified bone matrix, autologous bone, MICROS superfine mill, bony fusion, bone graft substitute, osteoblasts, osteogenic induction, provincial grants-supported paper

CLC Number:

R318

Huang Kai, Chen Xiong-sheng, Jia Lian-shun, Zhu Wei, Fang Lei. Nano decalcified bone matrix promotes bone fusion[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.21.009.

Figures/Tables 9

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