Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (21): 3057-3064.doi: 10.3969/j.issn.2095-4344.2016.21.003

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Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) scaffolds carrying human bone marrow mesenchymal stem cells for bone tissue engineering

Zhang Jun-biao1, 2, 3, He Zhi-xu2, 4, Ye Chuan2, 5, Wang Yong3, 6, Wang Mei7, Liu Qin2, 3, Yang Long2, Li Jing2, Ma Min-xian3, 6   

  1. 1Guizhou Medical University, Guiyang 550004, Guizhou Province, China; 2National and Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique, 3School of Stomatology, 4Department of Pediatrics, Guizhou Medical University, Guiyang 550004, Guizhou Province, China; 5Department of Orthopedics, 6Department of Stomatology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China; 7Guiyang Stomatological Hospital, Guiyang 550004, Guizhou Province, China
  • Received:2016-03-09 Online:2016-05-20 Published:2016-05-20
  • Contact: Ma Min-xian, Associate chief physician, School of Stomatology, Guizhou Medical University, Guiyang 550004, Guizhou Province, China; Department of Stomatology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China
  • About author:Zhang Jun-biao, Studying for master’s degree, National and Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique, School of Stomatology, Guizhou Medical University, Guiyang 550004, Guizhou Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81360232; the Science and Technology Joint Foundation of Guizhou, No. 2012002; the Science and Technology Project of Guiyang, No. 20141001.

Abstract:

BACKGROUND: As a noticeable tissue engineering material of polyhydroxyalkanoates family, poly(3-hydroxybutyrate-co-4-hydroxybutyrate)(P3HB4HB)exhibits good biocompatibility, adhesion and mechanical properties, presenting a extensive application future in tissue-engineered research.

 
OBJECTIVE: To investigate the biocompatibility in vitro and ectopic osteogenic differentiation in vivo of P3HB4HB and human bone marrow mesenchymal stem cells.
METHODS: Passage 5 human bone marrow mesenchymal stem cells transplanted onto the three-dimensional P3HB4HB scaffold were incubated with osteogenic induction medium (test group) or with no osteogenic induction(control group), respectively. After 5-day incubation, the cell growth was assessed by acridine orange staining and scanning electron microscopy; after14-day incubation, both kinds of cell-scaffold composites were subcutaneously implanted into the nude mice. At 16 weeks after implantation, the cell-scaffold composites were removed to observe ectopic osteogenic differentiation in vivo using hematoxylin-eosin staining, von Kossa staining and collagen type I immunohistochemical staining.

RESULTS AND CONCLUSION: Acridine orange staining showed that cells adhered well on the surface of the scaffold; under the scanning electron microscope, induced cells grew well on the P3HB4HB scaffold and produced abundant extracellular matrixes. In addition, at 16 weeks after implantation, there were osteoid tissues in the test group, positive for von Kossa staining as well as collagen type I immunohistochemical staining; furthermore, hematoxylin-eosin staining showed numerous osteoblasts and bone lacunas. In contrast, no bone tissues appeared in the control group. To conclude, P3HB4HB is a suitable material for bone tissue engineering.

 

 

 

Key words: Bone Marrow, Mesenchymal Stem Cells, 3-Hydroxybutyric Acid, Tissue Engineering

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