Bone marrow mesenchymal stem cells for bone nonunion under micro-damage environment

doi:10.3969/j.issn.2095-4344.2015.41.021

Abstract

Abstract:

BACKGROUND: Bone marrow stem cells combined with traditional surgery regimen can significantly improve the therapeutic effects on bone nonunion, which are considered to have an important application value.
OBJECTIVE: To explore therapeutic effect of bone marrow mesenchymal stem cells on bone nonunion under micro-damage environment.
METHODS: Forty New Zealand white rabbits were selected and randomized into experimental and control groups, 20 rabbits in each group. Bone marrow of the tibia was extracted to isolate and culture bone marrow mesenchymal stem cells. Passage 3 cells with the order of magnitudes of 107 were labeled by superparamagnetic iron oxide nanoparticles. A 15-mm bone defect was made at the middle of the radius of the rabbit forelimb. Bone nonunion appeared at 6 weeks after bone defects. Bone marrow mesenchymal stem cells combined with iliac particles were implanted into the bone defect of rabbits in the experimental group, and only iliac particles were implanted into the bone defect of rabbits in the control group. Within 12 weeks after implantation, the bone nonunion was observed through gross morphology, X-ray observation, and pathological observation.
RESULTS AND CONCLUSION: After implantation, a remarkable callus was found in the experimental group, and the bone defect recovered gradually until it was completely healed; in the control group, there was no callus,
and the bone marrow cavity was closed and full of granulation tissues. In the experimental group, there were actively proliferated cartilage tissues, bone particles were fused, osteoid structures appeared, and osteoblasts proliferated progressively; in the control group, poor cartilage hyperplasia was found, and there were a large amount of dead bone tissues but no fused bone particles and osteoblasts. In the experimental group, X-ray films on the defected radium showed cloudiness-like shadow, the bone marrow cavity was recanalized, and the skeleton was shaped well; in the control group, few bone particles were absorbed, the bone marrow cavity was partly recanalized, and the injured bone was not healed with osteosclerosis. These findings indicate that under the micro-damage environment, bone marrow mesenchymal stem cells can differentiate into osteoblasts to repair bone defects-induced bone nonunion.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cell Transplantation, Radius, Bone Diseases, Tissue Engineering

Wang Yue-fu, Yu Xi-xin. Bone marrow mesenchymal stem cells for bone nonunion under micro-damage environment[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(41): 6677-6682.

Figures/Tables 5

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