Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (24): 3824-3831.doi: 10.3969/j.issn.2095-4344.2015.24.011

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Peritubular capillary network in 5/6 nephrectomized rats undergoing naked netrin-1 plasmid transfection 

Li Jian-feng1, Han Hong-guang2, Li Xiao-mi2, Shi Hong3   

  1. 1 Department of Medical Administration, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China
    2 ICU of Department of Cardiovascular Surgery, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China
    3 Department of Nephrology, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
  • Online:2015-06-11 Published:2015-06-11
  • Contact: Han Hong-guang, M.D., Associate chief physician, ICU of Department of Cardiovascular Surgery, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China
  • About author:Li Jian-feng, Associate chief physician, Department of Medical Administration, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China

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Abstract:

BACKGROUND: Renal tubular-interstitial lesion and fibrosis induced by peritubular capillary reduction is a common pathway for various chronic kidney diseases which eventually develop into end-stage renal failure. How to increase the density of peritubular capillary network is the key to resolving renal interstitial fibrosis. Netrin-1, as a potent mitogen of vascular endothelial cells, can promote the migration and proliferation of vascular endothelial cells and induce angiogenesis.     
OBJECTIVE: To observe the protective effects of naked netrin-1 plasmid transfer on the remnant renal function of 5/6 nephrectomized rats and the effects of naked netrin-1 plasmid transfer on peritubular capillary network, and to further discuss the possible mechanism.    
METHODS: Thirty Sprague-Dawley rats were randomly divided into sham, model and treatment groups. Upper and lower one-third of the rat left kidney was resected in the model and treatment groups, and then the right kidney was resected after 1 week to prepare remnant kidney models in rats. IRES2-EGFP and pCMV6-XL5-Netrin-1-IRES2-EGFP pGenesil-NPs plasmids were intravenously injected into the left renal vein while resecting the right kidney of rats in the model and treatment groups, respectively.
RESULTS AND CONCLUSION: Compared with model group, the levels of blood urea nitrogen and serum creatinine decreased, the degree of renal interstitial fibrosis alleviated, the density of peritubular capillaries increased, and the netrin-1 protein expression in renal tubular cytoplasm increased in the treatment group. These findings suggest that the naked netrin-1 plasmid transfer can significantly improve the renal function of the 5/6 nephrectomized rats, reduce the pathological lesion and renal interstitial fibrosis of the remnant kidney, increase the density of peritubular capillaries, and decrease the expression of hypoxia-inducible factor 1α, thereby improving the anoxic condition of renal interstitial tubules.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Plasmids, Kidney, Hypoxia-Inducible Factor 1, Transfection

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