Physicochemical properties of chitosan/bone powder/cellulose nanocrystals scaffold loaded with antimicrobial peptides for jaw repair

doi:10.12307/2023.412

Abstract

Abstract: BACKGROUND: It is difficult for a single biomaterial to reach the standard of an ideal scaffold material for bone tissue engineering. It is an effective way to construct an ideal scaffold by compounding and complementing the advantages of multiple biomaterials.
OBJECTIVE: To construct a new type of mandibular scaffold, and test the key physicochemical properties of the scaffold.
METHODS: Antimicrobial peptide-loaded carboxymethyl chitosan/bone powder/cellulose nanocrystals scaffold was prepared by freeze-drying method. The microstructure of the scaffold was observed and analyzed, and the tensile strength and elongation at break of the scaffold were measured. The water absorption, degradation rate in vitro, thermal stability and release of antimicrobial peptide of the scaffold were evaluated.
RESULTS AND CONCLUSION: (1) Scanning electron microscope showed that the scaffolds had a uniform porous network structure with an average pore size of 114. 9 μm. The water absorption rate of the scaffold was 698.8±53.8%. The fracture elongation of the scaffold was 8%-10%. The tensile strength was 0.7-0.9 MPa, and the stress-strain relationship was similar to that of natural cartilage tissue. (2) Thermogravimetric curve displayed that the weight loss of the stent was very slight below 200 °C, obvious between 200-400 °C, and severe above 600 °C. (3) The results of drug release in vitro showed that (58.40±1.79)% of antibacterial peptide was released from the scaffold within the first 12 hours, and the drug release rate decreased after 12 hours, and nearly 20% of antibacterial peptide was not released after 6 days, which was beneficial to the sustained and stable drug effect. (4) The scaffolds were separately soaked in PBS and PBS containing lysozyme. At 28 days, the degradation rates of scaffolds without lysozyme and with lysozyme were 70% and 85%, respectively. (5) These results exhibited that the carboxymethyl chitosan/bone powder/cellulose nanocrystals scaffold had good water absorption performance, thermal stability, degradation performance and mechanical property.

Key words: carboxymethyl chitosan, cellulose nanocrystals, hydroxyapatite, antimicrobial peptide, jawbone, scaffold, bone tissue engineering

CLC Number:

Yu Aimin, Xu Ting, Zhu Yunying, Liang Jianqiang, Wu Donghui. Physicochemical properties of chitosan/bone powder/cellulose nanocrystals scaffold loaded with antimicrobial peptides for jaw repair[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(25): 3999-4005.

Figures/Tables 8

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