Effect of total glucosides of paeony on inflammation and degeneration of chondrocytes in osteoarthritis

doi:10.12307/2021.158

Abstract

Abstract: BACKGROUND: Cartilage degeneration is one of the most characteristic pathological manifestations of osteoarthritis, which plays an important role in the pathogenesis of osteoarthritis. The total glucosides of paeony (TGP) is the effective components of white paeony root, which has strong anti-inflammatory, analgesic and immunomodulatory effects. However, its effect on inflammation and degeneration of cartilage is still unclear.
OBJECTIVE: To observe the effects of TGP on interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and matrix metalloproteinase-13 (MMP-13) in osteoarthritic chondrocytes in osteoarthritis.
METHODS: Chondrocytes were extracted from the cartilage tissue discarded by knee replacement surgery by collagenase digestion. The effects of different concentrations on the proliferation of chondrocytes were detected by cell counting kit-8 method. The cultured cells were further divided into five groups: blank group, lipopolysaccharide (10 mg/L) intervention group, low concentration TGP group (10 μmol/L TGP+10 mg/L lipopolysaccharide), medium concentration TGP group (20 μmol/L TGP+10 mg/L lipopolysaccharide) and high concentration TGP group (50 μmol/L TGP+10 mg/L lipopolysaccharide). Except for the normal group, the other four groups were induced with lipopolysaccharide to induce inflammation. Real-time quantitative PCR and western blot assay were used to detect the expression of IL-1β, TNF-α and MMP-13 in each group at 24 hours after intervention.
RESULTS AND CONCLUSION: TGP of 10, 20 and 50 μmol/L significantly stimulated the proliferation of chondrocytes, and excessive concentration of TGP significantly inhibited the proliferation. Chondrocytes with lipopolysaccharide induction significantly increased the expression of IL-1β, TNF-α and MMP-13 (P < 0.01), while TGP could significantly reduce IL-1β, TNF-α and MMP-13 expression in lipopolysaccharide-induced chondrocytes in a concentration-dependent manner (P < 0.05, P < 0.01). To conclude, TGP has an obvious anti-inflammatory effect and delays extracellular matrix loss in chondrocytes.

Key words: total glucosides of paeony, osteoarthritis, chondrocytes, interleukin-1β, tumor necrosis factor-α, matrix metalloproteinase-13

CLC Number:

Chen Pu, Ruan Anmin, Zhou Jun, Zhang Xiaozhe , Ma Yufeng, Peng Haoxuan, Yang Tongjie, Wang Qingpu. Effect of total glucosides of paeony on inflammation and degeneration of chondrocytes in osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(29): 4614-4618.

Figures/Tables 4

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