Effect of serum containing Bushen Tiaogan prescription on interleukin-1beta-induced chondrocyte apoptosis

doi:10.12307/2021.286

Abstract

Abstract: BACKGROUND: Previous studies have found that Bushen Tiaogan prescription can significantly improve the symptoms of osteoarthritis, with a positive clinical effect, but there is still a lack of relevant experimental evidence.
OBJECTIVE: To explore the effect of serum containing Bushen Tiaogan prescription on interleukin (IL)-1β-induced chondrocyte apoptosis and its molecular mechanism.
METHODS: Passage 3 chondrocytes from rats were randomly divided into four groups: PBS group (control group), 10 μg/L IL-1β group, 10 μg/L IL-1β+5% Bushen Tiaogan prescription group, 10 μg/L IL-1β+10% Bushen Tiaogan prescription group, in which chondrocytes were treated for 24 hours. Flow cytometry was used to detect cell apoptosis, and western blot was used to analyze the expression levels of SOX9, NF-κB P65 and p-NF-κB P65 proteins. An approval was obtained from the Animal Experimental Ethics Committee of Guangzhou University of Chinese Medicine.
RESULTS AND CONCLUSION: Compared with the control group, IL-1β induced chondrocyte apoptosis (P < 0.05). Compared with the IL-1β group, the apoptosis rate of chondrocytes in the 10 μg/L IL-1β+5% Bushen Tiaogan prescription and 10 μg/L IL-1β+10% Bushen Tiaogan prescription groups significantly decreased in a dose-dependent manner (P < 0.05). Compared with the control group, the expression of SOX9 in the IL-1β group was significantly lower than that of the control group (P < 0.05), and the expression of p-P65/P65 was significantly higher than that of the control group (P < 0.05). The expression of SOX9 in the 10 μg/L IL-1β+5% Bushen Tiaogan prescription and 10 μg/L IL-1β+10% Bushen Tiaogan prescription groups was significantly higher than that in the IL-1β group (P < 0.05), and the expression of p-P65/P65 in the 10 μg/L IL-1β+10% Bushen Tiaogan prescription group was significantly lower than that in the IL-1β group (P < 0.05). To conclude, Bushen Tiaogan prescription may inhibit IL-1β-induced apoptosis of rat osteoarticular chondrocytes by regulating SOX9/NF-κB, and play a role in alleviating articular cartilage degeneration.

Key words: osteoarthritis, Bushen Tiaogan prescription, articular cartilage, SOX9/NF-κB

CLC Number:

Fan Shuai, Lin Jiebin, Wu Chunfei, Xu Zhaohui. Effect of serum containing Bushen Tiaogan prescription on interleukin-1beta-induced chondrocyte apoptosis[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(35): 5594-5598.

Figures/Tables 4

References

[1] CROSS M, SMITH E, HOY D, et al. The global burden of hip and knee osteoarthritis: estimates from the global burden of disease 2010 study. Ann Rheum Dis. 2014; 73(7):1323-1330.
[2] 陆艳红, 石晓兵.膝骨关节炎国内外流行病学研究现状及进展[J].中国中医骨伤科杂志,2012,20(6):81-84.
[3] LEI Z, SHI H, LI W, et al. miR-185 inhibits non-small cell lung cancer cell proliferation and invasion through targeting of SOX9 and regulation of Wnt signaling. Mol Med Rep. 2018;17(1):1742-1752.
[4] KANG S, ZHANG J, YUAN Y. Abietic acid attenuates IL-1beta-induced inflammation in human osteoarthritis chondrocytes. Int immunopharmacol. 2018;64:110-115.
[5] WANG J, CHEN L, JIN S, et al. Altered expression of microRNA-98 in IL-1beta-induced cartilage degradation and its role in chondrocyte apoptosis. Mol Med Rep. 2017;16(3):3208-3216.
[6] HUANG Z, ZHOU M, WANG Q, et al. Mechanical and hypoxia stress can cause chondrocytes apoptosis through over-activation of endoplasmic reticulum stress. Arch Oral Biol. 2017;84:125-132.
[7] PAN T, CHEN R, WU D, et al. Alpha-Mangostin suppresses interleukin-1β-induced apoptosis in rat chondrocytes by inhibiting the NF-κB signaling pathway and delays the progression of osteoarthritis in a rat model. Int Immunopharmacol. 2017;52:156-162.
[8] ZHANG W, CHENG P, HU W, et al. Inhibition of microRNA-384-5p alleviates osteoarthritis through its effects on inhibiting apoptosis of cartilage cells via the NF-κB signaling pathway by targeting SOX9. Cancer Gene Ther. 2018;25(11-12):326-338.
[9] 吴春飞,易俊,梁桂洪,等.补肾调肝方治疗肝郁肾虚膝骨关节炎的临床研究[J].中医临床研究,2017,9(36):74-76.
[10] FELSON DT. Osteoarthritis of the knee. N Engl J Med. 2006;354(23):841-848.
[11] YANG S, DUBE CE, EATON CB, et al. Longitudinal use of complemen-tary and alternative medicine among older adults with radiographic knee osteoarthritis.Clin Therapeut. 2013;35(11):1690-1702.
[12] 杨建辉,吕建国,聂会勇,等.白藜芦醇对白细胞介素-1β诱导的软骨细胞凋亡及增殖能力的影响[J].中国老年学杂志,2014,34(21):6151-6153.
[13] JUNYI L, NING H, NIAN Z, et al. Sox9 Potentiates BMP2-Induced Chondrogenic Differentiation and Inhibits BMP2-Induced Osteogenic Differentiation. Plos One. 2014;9(2):e89025.
[14] SUN Q, WU G, CHEN H, et al. Hyperbaric oxygen protects type II collagen in interleukin-1β-induced mandibular condylar chondrocyte via inhibiting the JNK/c-Jun signaling pathway. Oncotarget. 2017;8(36):60312-60323.
[15] YAO B, WANG Q, LIU CF, et al. The SOX9 upstream region prone to chromosomal aberrations causing campomelic dysplasia contains multiple cartilage enhancers. Nucleic Acids Res. 2015;43(11):5394-5408.
[16] 汤晓晨,俞峰,孙书龙,等.黄芪甲苷对人膝骨关节炎退变关节软骨IL-1β表达的影响[J].南京中医药大学学报,2013,29(1):48-52.
[17] 姚丽,赵婧,周强,等.祛痰化瘀利湿方对大鼠膝骨关节炎滑膜组织和血清IL-1β,MMP-1及COMP,CTX-Ⅱ的影响[J].中国中医骨伤科杂志,2015,23(11):5-8.
[18] 贺牡丹,王小平,陈同生.白细胞介素-1β诱导关节软骨细胞凋亡的分子机理[J].中国细胞生物学学报,2011,33(1):49-54.
[19] GOLDRING MB, OTERO M, PLUMB DA, et al.Roles of inflammatory and anabolic cytokines in cartilage metabolism: signals and multiple effectors converge upon MMP-13 regulation in osteoarthritis. Eur Cell Mater. 2011;21(24):202-220.
[20] 季卫锋,童培建,袁小凤,等.补肾法与活血法对SD大鼠膝骨性关节炎滑膜IL-1β、TNF-α及软骨MMP-13、ADAMTS-5的影响[J].中国中医骨伤科杂志, 2012,20(2):1-5.
[21] ZHENG W, ZHANG H, JIN Y, et al. Butein inhibits IL-1β-induced inflammatory response in human osteoarthritis chondrocytes and slows the progression of osteoarthritis in mice. Int Immunopharmacol. 2017;42:1-10.
[22] LI W, ZHANG L, GUO B, et al. Exosomal FMR1-AS1 facilitates maintaining cancer stem-like cell dynamic equilibrium via TLR7/NFκB/c-Myc signaling in female esophageal carcinoma. Mol Cancer. 2019;18(1):22.
[23] RAO Z, WANG S, WANG J. Protective effects of psoralidin on IL-1β-induced chondrocyte apoptosis. Mol Med Rep. 2018;17(2):3418-3424.
[24] 罗珊,霍永鑫,刘英,等.连翘苷对IL-1β诱导的人关节软骨细胞凋亡及细胞外基质降解的影响[J].免疫学杂志,2019,35(8):645-652.
[25] LIU YX, WANG GD, WANG X, et al. Effects of TLR-2/NF-κB signaling pathway on the occurrence of degenerative knee osteoarthritis: an in vivo and in vitro study. Oncotarget. 2017;8(24):38602-38617.
[26] JEONG JW,LEE HH,LEE KW,et al. Mori folium inhibits interleukin-1beta-induced expression of matrix metalloproteinases and inflammatory mediators by suppressing the activation of nf- kappab and p38 mapk in sw1353 human chondrocytes. Int J Mol Med. 2016;37(2):452-460.
[27] YU G, WAN R, YIN G, et al. Diosmetin ameliorates the severity of cerulein-induced acute pancreatitis in mice by inhibiting the activation of the nuclear factor-kappa B. Int J Clin Exp Pathol. 2014;7(5):2133-2142.
[28] JEONG JW, LEE HH, CHOI EO, et al. Schisandrae fructus inhibits il- 1beta-induced matrix metalloproteinases and inflammatory mediators production in sw1353 human chondrocytes by suppressing NF-kappa B and mapk activation. Drug Dev Res. 2015;76(8):474-483.
[29] YANG Y, GONG XB, HUANG LG, et al. Diosmetin exerts anti- oxidative,anti-inflammatory and anti-apoptotic effects to protect against endotoxin-induced acute hepatic failure in mice. Oncotarget. 2017;8(19):30723-30733.
[30] 鲁宏, 宋俊, 孟京红,等. 香叶木素通过NF-κB通路缓解IL-1β诱导的新生大鼠骨关节炎软骨细胞凋亡和免疫反应[J].中国免疫学杂志,2019,35(3):292-297.