Osteogenic induction and identification of mammary stem cells derived from a Holstein dairy cow

doi:10.3969/j.issn.2095-4344.1643

Abstract

Abstract:

BACKGROUND: The isolated and purified mammary stem cells from Holstein dairy cows have been induced to differentiate into nerve cells, adipocytes and osteoblasts, further enriching their multi-directional differentiation potential, which is of great significance for the study on mammary stem cells.
OBJECTIVE: To investigate the osteogenic potential of mammary stem cells from Holstein dairy cows.
METHODS: Mammary stem cells from Holstein dairy cows were isolated and purified, which were provided by the Key Laboratory of Biological Manufacturing of Inner Mongolia Autonomous Region. The fourth generation of mammary stem cells were cultured for 21 days in an osteogenic induction medium, and identified by alizarin red staining and RT-PCR.
RESULTS AND CONCLUSION: After osteogenic induction, the cell edge was indistinct, and there were some granular calcified nodules in the cells. The induced cells were positive for alizarin red staining. The positive expressions of Alpl and Bglap in the induced osteoblasts were detected, indicating that the mammary stem cells from Holstein dairy cows have the potential to differentiate into osteoblasts.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Cattle, Mammary Glands, Animal, Stem Cells, Cell Differentiation, Osteoblasts, Tissue Engineering

CLC Number:

Chen Yumeng, Han Zhexian, Liu Yingchun, Gao Feng. Osteogenic induction and identification of mammary stem cells derived from a Holstein dairy cow[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(9): 1359-1363.

Figures/Tables 4

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