Mechanical properties and cytocompatibility of a new-type nano-bionic anti-adhesion hernia mesh

doi:10.3969/j.issn.2095-4344.0066

Abstract

Abstract:

BACKGROUND: A new-type nano-bionic anti-adhesion hernia mesh was developed in our previous research.

OBJECTIVE: To investigate the mechanical properties and cytocompatibility of the new-type nano-bionic anti-adhesion hernia mesh.

METHODS: The tensile strength of the compound hernia mesh was detected using a textile detector. Mouse fibroblasts (L929) were cultured with the compound hernia mesh, and cell structures on the mesh surface were observed under electron microscope at 1, 3, 5 days after culture. In addition, L929 cells were co-cultured with compound hernia mesh, polypropylene patch, and polyester patch, respectively. Cells cultured alone were used as negative controls. After 1, 3, 5 days of culture, MTS array was used to detect cell proliferation and evaluate cytotoxicity; after 3 days of culture, western blot was used to detect the content of type I and III collagens.

RESULTS AND CONCLUSION: The average tensile strength of the compound hernia mesh was 31.2 N. The number of fibroblasts on the nanofibrous layer of the compound hernia mesh increased as long as cultured. The cells spread along the nanofibers and pseudopodia extended from the cells formed polygon and fusiform structures, with a good cross-linking with the mesh. A complete cell layer covered all pores of the nanofibers at 5 days. The cytotoxicity of the nanofibrous layer of the compound hernia mesh was graded 0, and the cytotoxicity was graded 1 of polypropylene and polyester patches. All the three kinds of patches fulfilled the implantation requirements, and the compound hernia mesh had better biological properties. No significant differences were found among groups in the contents of type I and III collagens at 3 days of culture. To conclude, the new-type nano-bionic anti-adhesion hernia mesh has good mechanical properties and cytocompatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Nanofibers, Polypropylenes, Hernia, Fibroblasts, Tensile Strength, Tissue Engineering

CLC Number:

R318

Tang Xiao, Ye Xiao-long, Huang Jiang-long, Yang Xiao-feng, Zheng Zong-heng, Wei Bo, Chen Tu-feng, Huang Yong, Luo Lin-bo, Zhan Ze-feng, Wei Hong-bo. Mechanical properties and cytocompatibility of a new-type nano-bionic anti-adhesion hernia mesh[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(6): 902-907.

Figures/Tables 8

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