Three kinds of mesenchymal stem cells: differentiating into neuron-like cells

doi:10.3969/j.issn.2095-4344.0671

Abstract

Abstract:

BACKGROUND: Stem cells, characterized with convenient collection, wide sources and great potential, have been developed in the cell therapy. The most commonly used stem cells include bone marrow mesenchymal stem cells, placental mesenchymal stem cells and umbilical cord blood mesenchymal stem cells.
OBJECTIVE: To compare the biological changes in bone marrow mesenchymal stem cells, placental mesenchymal stem cells and umbilical cord blood mesenchymal stem cells after co-cultured with nerve cells.
METHODS: Human bone marrow mesenchymal stem cells, human placental mesenchymal stem cells and human umbilical cord blood mesenchymal stem cells were isolated and cultured in vitro. The morphological changes of mesenchymal stem cells differentiated into nerve cells were observed by co-culture with Transwell culture plates. The expression of neuron-specific enolase was detected after induction.
RESULTS AND CONCLUSION: In the co-culture system, these three kinds of mesenchymal stem cells gradually formed a star-like shape and were interconnected. Moreover, all the cells were positive for neuron-specific enolase. These findings reveal that the microenvironment provided by nerve cells can induce and promote the differentiation of three kinds of mesenchymal stem cells into neuron-like cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Mesenchymal Stem Cells, Coculture Techniques, Tissue Engineering

CLC Number:

R394.2

Yang Ying, Xu Qian, Wang Hui-ling, Yao Min-xiu. Three kinds of mesenchymal stem cells: differentiating into neuron-like cells[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(33): 5356-5361.

Figures/Tables 2

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