Effect of magnetic labeled endothelial progenitor cell transplantation on renal function of diabetic rats: a MRI imaging verification

doi:10.3969/j.issn.2095-4344.2017.05.010

Abstract

Abstract:

BACKGROUND: Endothelial progenitor cells have been shown to play an important role in the pathogenesis of traumatic diseases in recent years.
OBJECTIVE: To explore the effect of magnetic labeled endothelial progenitor cell transplantation on renal
function of diabetic rats through a MRI imaging study.
METHODS: Sixty Wistar rats were randomly divided into normal (no treatment), control and experimental groups. Intraperitoneal injection of 40 mg/kg streptozotocin was performed to make a rat model of type 1 diabetes in the control and experimental groups. Four weeks after modeling, rats in the experimental group were given intravenous injection of magnetic labeled endothelial progenitor cells (0.15 mL, 1×10⁹/L). Fasting blood glucose, serum insulin, serum creatinine, urea nitrogen and 24-hour urinary protein levels in rats were measured at 8 weeks after cell transplantation. MRI was used to trace transplanted cells in vivo in comparison with renal biopsy findings, and rat body mass and kidney weight were measured to calculate kidney weight index.
RESULTS AND CONCLUSION: After modeling, fasting blood glucose, serum creatinine, urea nitrogen and 24-hour urinary protein levels as well as kidney weight index were increased significantly (P < 0.05), while the insulin level decreased (P < 0.05). Compared with the model group, the endothelial progenitor cell transplantation reversed these indices (P < 0.05). Additionally, in the experimental group, there was slightly longer T1 and shorter T2 signals as well as marked lesion edge, and the FLASH sequence became more remarkable compared with the T2-weighted RARE sequence. The other groups showed no significant low signal changes. Magnetic-labeled positive cells in the experimental group showed by the MRI were consistent with the tissue biopsy results, while no positive cells were found in the model and normal groups. To conclude, the magnetic labeled endothelial progenitor cell transplantation can improve renal dysfunction in diabetic rats to a certain extent.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Diabetes Mellitus, Tissue Engineering

CLC Number:

R394.2

Feng Na, Xu Ying-jin, Dong Xi, Yang Jing-jing, He Xin. Effect of magnetic labeled endothelial progenitor cell transplantation on renal function of diabetic rats: a MRI imaging verification[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(5): 713-717.

Figures/Tables 3

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