Identification of genomic full-length sequence of human leukocyte antigen-E and its two novel alleles

doi:10.3969/j.issn.2095-4344.2017.25.022

Abstract

Abstract:

BACKGROUND: Human leukocyte antigen-E (HLA-E) is one of non-classical HLA class I genes. Up to now, the polymorphism analysis is mainly aimed at the variation in exon 3 of HLA-E, which determines HLA-E*01:01 or HLA-E*01:03. However, the identification of the full-length HLA-E and its novel alleles is rare reported.
OBJECTIVE: To establish the method of identification of HLA-E genomic full-length sequence, and to identify its novel alleles in healthy blood donors in Shenzhen, China.
METHODS: Peripheral blood DNA samples were extracted from the subjects, and the amplified primers and sequencing primers in conserved regions were designed according to the sequences of HLA-E published in the IMGT/HLA database. A high-fidelity reaction system was used to amplify the genomic full-length of HLA-E, followed by sequencing, assembling, confirming and typing.
RESULTS AND CONCLUSION: Herein, we successfully established the method for amplifying genomic full-length sequence and sequence-based typing. Two novel HLA-E alleles were nominated by WHO HLA Nomenclature committee as HLA-E*01:01:01:06 and HLA-E*01:01:01:07. Compared with the most related allele HLA-E*01:01:01:01, HLA-E*01:01:01:06 had one nucleotide change at nt-26(G->T) in 5’-promoter, and HLA-E*01:01:01:07 had one nucleotide change at nt3345(T->C) in 3’-UTR. The polymorphism data of genomic full-length HLA-E in Chinese individuals need to be filled, and the method we developed here supplies the key technique for the further studies.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: HLA Antigens, Alleles, Tissue Engineering

CLC Number:

R394.2

He Liu-mei, Wang Song-xing, Xu Yun-ping. Identification of genomic full-length sequence of human leukocyte antigen-E and its two novel alleles[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(25): 4068-4074.

Figures/Tables 4

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