Collagen-gelatin scaffolds for the repair of peripheral nerve defects

Abstract

Abstract:

BACKGROUND: Collagen-gelatin composite scaffolds have been reported to be able to promote the early recovery of peripheral nerve defects. However, this conclusion has not been further confirmed.
OBJECTIVE: To investigate the biocompatibility of the collagen-gelatin scaffold and its treatment outcomes in the repair of peripheral nerve defects.
METHODS: The collagen-gelatin scaffold was co-cultured with bone marrow mesenchymal stem cells (BMSCs) of Sprague-Dawley rats for 5 days, and then the cell growth was observed. Twenty Sprague-Dawley rats were enrolled, modeled into a left 30-mm peroneal nerve defect and randomized into experimental and control groups. The collagen-gelatin scaffold composited with BMSCs was implanted into the experimental group, and autograft
nerve implanted into the control group. Morphology of the middle bridge was observed, and electrophysiology was conducted at 16 weeks after implantation.
RESULTS AND CONCLUSION: BMSCs grew and adhered well onto the scaffold. The myelinated nerve fiber density did not significantly differ between groups (P > 0.05). The myelinated nerve fiber diameter, myelin sheath thickness and percentage of nerve tissues in the experimental group were significantly lower than those in the control group (P < 0.05). There were no significant differences in the conduction velocity, latency of motor nerves and the conduction velocity and amplitude of sensory nerves between groups (P > 0.05). The amplitude of motor nerves and the latency of sensory nerves in the experimental group were significantly lower than those in the control group (P < 0.05). To conclude, the collagen-gelatin scaffold holds a good cytocompatibity and is ideal for the repair of peripheral nerve defects.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Collagen, Gelatin, Peripheral Nerves, Tissue Engineering

CLC Number:

R318

Liu Tian-dan, Zhang Bao-chao, Hao Ming-liang.

Collagen-gelatin scaffolds for the repair of peripheral nerve defects

[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(2): 286-290.

Figures/Tables 6

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