Hollow hydroxyapatite combined with human bone morphogenetic protein-2 microspheres for bone defect repair

doi:10.3969/j.issn.2095-4344.2017.02.003

Abstract

Abstract:

BACKGROUND: Hydroxyapatite (HA) is a good scaffold material, and recombinant human bone morphogenetic protein-2 (rhBMP-2) possesses a strong osteogenic ability, therefore, by which preparing a novel composite material will be helpful for bone repair.
OBJECTIVE: To explore the effects of the hollow HA/rhBMP-2 microspheres on the osteogenesis and biomechanics of rabbit bone defects.
METHODS: Forty-eight male healthy adult New Zealand white rabbits were randomly divided into three groups (n=16 per group), including composite, single and control groups. Radical defect models were prepared, and the hollow HA/rhBMP-2 and hollow HA scaffolds were implanted into the composite and single groups, respectively. The control group received no treatment. At the 1st day of 4, 8, 12, and 16 weeks after implantation, the level of serum alkaline phosphatase was detected, and the bone healing was assessed through X-ray, three-dimensional CT, radionuclide bone scan and biomechanics testing, respectively.
RESULTS AND CONCLUSION: The level of serum alkaline phosphatase, X-ray scale scores, osteogetic effect, region of interest volume, three-dimensional CT and biomechanical strength in the composite group were superior to those in the single group. In the meanwhile, the bone healing was unsatisfactory in the control group. Our findings indicate that the hollow HA/rhBMP-2 artificial bone exhibits a good osteogenic ability and mechanical strength, contributing to bone healing.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Radius Fractures, Hydroxyapatites, Bone Morphogenetic Proteins, Tissue Engineering

CLC Number:

R318

Tang Zhi-ming, Xiong Long, Zeng Jian-hua, Liao Xin-gen, Li Jing-tang, Yao Ai-hua, Hua Fu-zhou. Hollow hydroxyapatite combined with human bone morphogenetic protein-2 microspheres for bone defect repair[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(2): 177-181.

Figures/Tables 4

References

[1]Xiao W, Fu H, Rahaman MN, et al. Hollow hydroxyapatite microspheres: a novel bioactive and osteoconductive carrier for controlled release of bone morphogenetic protein-2 in bone regeneration. Acta Biomater. 2013;9(9):8374-8383.

[2]Fu J, Yamamoto A, Kim HY, et al. Novel Ti-base superelastic alloys with large recovery strain and excellent biocompatibility. Acta Biomater. 2015;17:56-67.

[3]Silber JS, Anderson DG, Daffner SD, et al. Donor site morbidity after anterior iliac crest bone harvest for single-level anterior cervical discectomy and fusion. Spine (Phila Pa 1976). 2003;28(2):134-139.

[4]Liao Z, Wang CH, Cui WL. Comparison of Allograft and Autograft in Lumbar Fusion for Lumbar Degenerative Diseases: A Systematic Review. J Invest Surg. 2016;29(6): 373-382.

[5]Fu H, Rahaman MN, Brown RF, et al. Evaluation of bone regeneration in implants composed of hollow HA microspheres loaded with transforming growth factor β1 in a rat calvarial defect model. Acta Biomater. 2013;9(3): 5718-5727.

[6]Green DW, Padula MP, Santos J, et al. A therapeutic potential for marine skeletal proteins in bone regeneration. Mar Drugs. 2013;11(4):1203-1220.

[7]Fei Y, Gronowicz G, Hurley MM. Fibroblast growth factor-2, bone homeostasis and fracture repair. Curr Pharm Des. 2013; 19(19):3354-3363.

[8]Haubold M, Weise A, Stephan H, et al. Bone morphogenetic protein 4 (BMP4) signaling in retinoblastoma cells. Int J Biol Sci. 2010;6(7):700-715.

[9]Romagnoli C, D'Asta F, Brandi ML. Drug delivery using composite scaffolds in the context of bone tissue engineering. Clin Cases Miner Bone Metab. 2013;10(3):155-161.

[10]Hasegawa M, Doi Y, Uchida A. Cell-mediated bioresorption of sintered carbonate apatite in rabbits. J Bone Joint Surg Br. 2003;85(1):142-147.

[11]韩丽娜,于梦雪,刘学娜.羟基磷灰石的临床研究进展[J].中国医药指南,2015,13(20):45-47.

[12]Huber FX, Belyaev O, Hillmeier J, et al. First histological observations on the incorporation of a novel nanocrystalline hydroxyapatite paste OSTIM in human cancellous bone. BMC Musculoskelet Disord. 2006 8;7:50.

[13]姚爱华,艾凡荣,刘欣,等.硼酸盐玻璃转化制备中空羟基磷灰石微球的研究[J].无机材料学报,2010,25(1):53-57.

[14]姚爱华,徐为,艾凡荣,等.中空羟基磷灰石微球作为rhBMP-2缓释载体的研究[J].无机材料学报,2011,26(9):974-978.

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