Hepatic failure patient’s serum before and after plasmapheresis induces the differentiation of umbilical cord mesenchymal stem cells into hepatic cells

doi:10.3969/j.issn.2095-4344.2017.17.006

Abstract

Abstract:

BACKGROUND: Umbilical cord mesenchymal stem cells (UC-MSCs) have made certain curative effect on hepatic failure, but little is reported on the effect of hepatic failure patient’s serum microenvironment on UC-MSCs differentiation ability.
OBJECTIVE: To investigate the effect of hepatic failure patient’s serum on the differentiation of umbilical cord mesenchymal stem cells into hepatic cells.
METHODS: UC-MSCs were isolated by tissue adherent method and the cell morphology and phenotype identified by microscope and flow cytometry. Alpha-MEM media with serum before/after plasmapheresis were used to culture the third generation of UC-MSCs, and regular fetal bovine serum culture medium acted as control group. Inverted microscope was used to observe the cell morphology in three groups. Immunohistochemical method was used to measure expression level of alpha fetoprotein, albumin and cytokeratin 18.
RESULTS AND CONCLUSION: A great amount of high-purity UC-MSCs could be obtained using tissue adherent method, which highly expressed CD44, CD73, CD90, CD10, but did not express CD45. Hepatic failure patient’s serum could change the morphology of UC-MSCs and induce UC-MSCs to express alpha fetoprotein, albumin and cytokeratin 18. The positive expression of alpha fetoprotein, albumin and cytokeratin 18 was significantly increased after plasmapheresis (P < 0.05). To conclude, hepatic failure patient’s serum after plasmapheresis exert more benefits to induce UC-MSCs to differentiate into hepatic cells than that before plasmapheresis.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Umbilical Cord, Mesenchymal Stem Cells, Liver Failure, Plasma Exchange, Cell Differentiation

CLC Number:

R394.2

Wang Jing-bo, Geng Da-ying, Li Xiao-ying, Chen Li, Xu Feng, Shi Zhao-zhang. Hepatic failure patient’s serum before and after plasmapheresis induces the differentiation of umbilical cord mesenchymal stem cells into hepatic cells[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(17): 2659-2664.

Figures/Tables 2

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