Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (1): 122-127.doi: 10.3969/j.issn.2095-4344.2017.01.022

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Directional differentiation of human amniotic mesenchymal stem cells into osteoblasts, chondrocytes and lipocytes in vitro

Li Yu-wan, Zhu Xi-zhong, Jin Ying, Wang Sheng-min, Yang Ji-bin, Xiong Hua-zhang, Zou Gang, Liu Yi   

  1. Department of Orthopedics, the Affiliated Hospital of Zunyi Medical College, Zunyi 563000, Guizhou Province, China
  • Online:2017-01-08 Published:2017-03-15
  • Contact: Liu Yi, Professor, Master’s supervisor, Department of Orthopedics, the Affiliated Hospital of Zunyi Medical College, Zunyi 563000, Guizhou Province, China
  • About author:Li Yu-wan, Studying for master’s degree, Department of Orthopedics, the Affiliated Hospital of Zunyi Medical College, Zunyi 563000, Guizhou Province, China
  • Supported by:

    the Science & Technology Program of Guizhou Province, No. SY [2010]3091

Abstract:

BACKGROUND: Human amniotic mesenchymal stem cells (hAMSCs) are a kind of adult stem cells that can differentiate into osteocytes, chondrocytes and lipocytes under different conditions in vitro. Compared with other mesenchymal stem cells (MSCs), hAMSCs have many advantages like wide source, noninvasive isolation, low immunogenicity and short growth cycle, making hAMSCs an important source of seed cells in tissue engineering.
OBJECTIVE: To investigate whether hAMSCs have the characteristics of MSCs and the ability of differentiation 
into osteoblasts, chondrocytes and lipocytes.
METHODS: Agreed by patients, the amniotic membrane from partus maturus parturients was separated, and hAMSCs were isolated by enzyme digestion. Passage 3 cells were cultured in osteogenic, chondrogenic or adipogenic medium induction.
RESULTS AND CONCLUSION: The alizarin red staining results showed multiple mineralized nodules after osteogenic induction, and the activity of alkaline phosphatase was significantly increased after induction. The toluidine blue staining showed positive results after chondrogenic induction, and a large amount of glycosaminoglycans were secreted from the cell matrix. After adipogenic differentiation, lipid droplets were observed in the cytoplasm of the cells shown by the oil red O staining. Real-time PCR results showed the mRNA expressions of relative genes at day 21 after osteogenic, chondrogenic and adipogenic induction upregulated significantly compared with the results at day 7 (P < 0.05). These experimental results demonstrate that hAMSCs have the basic characteristics of MSCs and possess the potential of differentiation into osteoblasts, chondrocytes and lipocytes, which could be selected as the seed cells for tissue engineering.

 

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Cell Differentiation, Amnion, Tissue Engineering

CLC Number: