Chitosan-gelatin sponge versus umbilical cord mesenchymal stem cellsfor skin wound healing

doi:10.3969/j.issn.2095-4344.2016.34.019

Abstract

Abstract:

BACKGROUND: Umbilical cord mesenchymal stem cells possess multipotent differentiation and self-replication abilities, and can differentiate into osteoblasts, chondrocytes, smooth muscle cells and vascular endothelial cells, which contribute to reepithelialization and angiogenesis.
OBJECTIVE: To compare effects of umbilical cord mesenchymal stem cells and chitosan-gelatin sponge in skin wound healing.
METHODS: Thirty male Sprague-Dawley rats were equivalently randomized into model, chitosan-gelatin sponge and stem cell groups. 3×3 cm full-thickness skin defects were created on the rat dorsal area in each group, and 1 day later, model rats in each group were given intradermal injection of PBS, chitosan gel or umbilical cord mesenchymal stem cells, respectively, for successive 15 days.
RESULTS AND CONCLUSION: Compared with the model group, the wound healing rate was higher, the epidermal thickness was decreased, and the collagen fiber area was larger in the chitosan-gelatin sponge and stem cell groups. Furthermore, the healing effect in the chitosan-gelatin sponge group was superior to that in the stem cell group. To conclude, both chitosan-gelatin sponge and umbilical cord mesenchymal stem cells, especially umbilical cord mesenchymal stem cells, can effectively repair skin defects.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Skin, Wound Healing, Tissue Engineering

CLC Number:

R318

Fang Shao-xia, Guo Fen. Chitosan-gelatin sponge versus umbilical cord mesenchymal stem cellsfor skin wound healing[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(34): 5136-5141.

Figures/Tables 3

References

[1] Wu Y, Huang S, Enhe J, et al. Insights into bone marrow-derived mesenchymal stem cells safety for cutaneous repair and regeneration. Int Wound J. 2012; 9(6):586-594.
[2] Falanga V. Wound healing and its impairment in the diabetic foot. Lancet. 2005;366(9498):1736-1743.
[3] Shaw TJ, Martin P. Wound repair at a glance. J Cell Sci. 2009;122(Pt 18):3209-3213.
[4] Tario JD Jr, Muirhead KA, Pan D, et al. Tracking immune cell proliferation and cytotoxic potential using flow cytometry. Methods Mol Biol. 2011;699:119-164.
[5] Brissett AE, Hom DB. The effects of tissue sealants, platelet gels, and growth factors on wound healing. Curr Opin Otolaryngol Head Neck Surg. 2003;11(4): 245-250.
[6] Efron PA, Moldawer LL. Cytokines and wound healing: the role of cytokine and anticytokine therapy in the repair response. J Burn Care Rehabil. 2004;25(2): 149-160.
[7] Alemdaro?lu C, De?im Z, Celebi N, et al. An investigation on burn wound healing in rats with chitosan gel formulation containing epidermal growth factor. Burns. 2006;32(3):319-327.
[8] Alsarra IA. Chitosan topical gel formulation in the management of burn wounds. Int J Biol Macromol. 2009;45(1):16-21.
[9] Hocking AM, Gibran NS. Mesenchymal stem cells: paracrine signaling and differentiation during cutaneous wound repair. Exp Cell Res. 2010;316(14): 2213-2219.
[10] Wu Y, Chen L, Scott PG, et al. Mesenchymal stem cells enhance wound healing through differentiation and angiogenesis. Stem Cells. 2007;25(10):2648-2659.
[11] Goldman R. Growth factors and chronic wound healing: past, present, and future. Adv Skin Wound Care. 2004; 17(1):24-35.
[12] Akela A, Nandi SK, Banerjee D, et al. Evaluation of autologous bone marrow in wound healing in animal model: a possible application of autologous stem cells. Int Wound J. 2012;9(5):505-516.
[13] Chen M, Przyborowski M, Berthiaume F. Stem cells for skin tissue engineering and wound healing. Crit Rev Biomed Eng. 2009;37(4-5):399-421.
[14] Luo G, Cheng W, He W, et al. Promotion of cutaneous wound healing by local application of mesenchymal stem cells derived from human umbilical cord blood. Wound Repair Regen. 2010;18(5):506-513.
[15] Amado LC, Saliaris AP, Schuleri KH, et al. Cardiac repair with intramyocardial injection of allogeneic mesenchymal stem cells after myocardial infarction. Proc Natl Acad Sci U S A. 2005;102(32):11474-11479.
[16] Li Y, Chen J, Zhang CL, et al. Gliosis and brain remodeling after treatment of stroke in rats with marrow stromal cells. Glia. 2005;49(3):407-417.
[17] Caplan AI, Dennis JE. Mesenchymal stem cells as trophic mediators. J Cell Biochem. 2006;98(5): 1076-1084.
[18] Sasaki M, Abe R, Fujita Y, et al. Mesenchymal stem cells are recruited into wounded skin and contribute to wound repair by transdifferentiation into multiple skin cell type. J Immunol. 2008;180(4):2581-2587.
[19] Seifert AW, Monaghan JR, Voss SR, et al. Skin regeneration in adult axolotls: a blueprint for scar-free healing in vertebrates. PLoS One. 2012; 7(4):e32875.
[20] Qi Y, Jiang D, Sindrilaru A, et al. TSG-6 released from intradermally injected mesenchymal stem cells accelerates wound healing and reduces tissue fibrosis in murine full-thickness skin wounds. J Invest Dermatol. 2014;134(2):526-537.
[21] Mohd Zohdi R, Abu Bakar Zakaria Z, Yusof N, et al. Gelam (Melaleuca spp.) Honey-Based Hydrogel as Burn Wound Dressing. Evid Based Complement Alternat Med. 2012;2012:843025.
[22] Hengartner NE, Fiedler J, Schrezenmeier H, et al. Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma. PLoS One. 2015;10(1):e0116772.
[23] Zhao J, Hu L, Gong N, et al. The effects of macrophage-stimulating protein on the migration, proliferation, and collagen synthesis of skin fibroblasts in vitro and in vivo. Tissue Eng Part A. 2015;21(5-6): 982-991.
[24] Louis KS, Siegel AC. Cell viability analysis using trypan blue: manual and automated methods. Methods Mol Biol. 2011;740:7-12.
[25] Hsu SH, Hsieh PS. Self-assembled adult adipose-derived stem cell spheroids combined with biomaterials promote wound healing in a rat skin repair model. Wound Repair Regen. 2015;23(1):57-64.
[26] Fu X, Fang L, Li X, et al. Enhanced wound-healing quality with bone marrow mesenchymal stem cells autografting after skin injury. Wound Repair Regen. 2006;14(3):325-335.
[27] Sunay O, Can G, Cakir Z, et al. Autologous rabbit adipose tissue-derived mesenchymal stromal cells for the treatment of bone injuries with distraction osteogenesis. Cytotherapy. 2013;15(6):690-702.
[28] Veronesi F, Maglio M, Tschon M, et al. Adipose-derived mesenchymal stem cells for cartilage tissue engineering: state-of-the-art in in vivo studies. J Biomed Mater Res A. 2014;102(7):2448-2466.
[29] Salem SA, Hwie AN, Saim A, et al. Human adipose tissue derived stem cells as a source of smooth muscle cells in the regeneration of muscular layer of urinary bladder wall. Malays J Med Sci. 2013;20(4): 80-87.
[30] Jeong HH, Piao S, Ha JN, et al. Combined therapeutic effect of udenafil and adipose-derived stem cell (ADSC)/brain-derived neurotrophic factor (BDNF)-membrane system in a rat model of cavernous nerve injury. Urology. 2013;81(5):1108.e7-14.
[31] Langer R, Vacanti JP. Tissue engineering. Science. 1993;260(5110):920-926.
[32] Jang CH, Cho YB, Choi CH, et al. Effect of umbilical cord serum coated 3D PCL/alginate scaffold for mastoid obliteration. Int J Pediatr Otorhinolaryngol. 2014;78(7):1061-1065.
[33] Hynes RO. The extracellular matrix: not just pretty fibrils. Science. 2009;326(5957):1216-1219.
[34] Lutolf MP. Integration column: artificial ECM: expanding the cell biology toolbox in 3D. Integr Biol (Camb). 2009;1(3):235-241.
[35] Frantz C, Stewart KM, Weaver VM. The extracellular matrix at a glance. J Cell Sci. 2010;123(Pt 24): 4195-4200.
[36] Lu H, Hoshiba T, Kawazoe N, et al. Cultured cell-derived extracellular matrix scaffolds for tissue engineering. Biomaterials. 2011;32(36):9658-9666.
[37] Choi KH, Choi BH, Park SR, et al. The chondrogenic differentiation of mesenchymal stem cells on an extracellular matrix scaffold derived from porcine chondrocytes. Biomaterials. 2010;31(20):5355-5365.
[38] Wang Y, Kim UJ, Blasioli DJ, et al. In vitro cartilage tissue engineering with 3D porous aqueous-derived silk scaffolds and mesenchymal stem cells. Biomaterials. 2005;26(34):7082-7094.