Indirect co-culture with endothelial progenitor cells improves proliferation and apoptosis of bone marrow mesenchymal stem cells of osteoporosis rats

doi:10.3969/j.issn.2095-4344.2016.14.003

Abstract

Abstract:

BACKGROUND: Previous studies have found that estrogen deficiency causes a reduction in the activity of bone marrow mesenchymal stem cells (BMSCs).
OBJECTIVE: To explore the effect of endothelial progenitor cells (EPCs) on the BMSCs proliferation and apoptosis ability of osteoporosis rats.
METHODS: Healthy female Sprague-Dawley rats, 6 weeks old, were enrolled and subjected to bilateral ovariectomy to make osteoporosis models. BMSCs and EPCs were isolated using density gradient centrifugation combined with adhesion method, and identified with surface markers, cell proliferation and immunocytochemistry in vitro. We used Transwell inserts to establish EPCs and OVX-BMSCs indirect co-culture system. Control groups were OVX-BMSCs group and sham-BMSCs group in which rats were only subjected to remove the equal amount of fat tissues around the ovary. Flow cytometry was applied to detect BMSCs proliferation and apoptosis ability.
RESULTS AND CONCLUSION: Compared with the control groups, the results of flow cytometry test showed that the proportion of OVX-BMSCs at S phase was significantly increased at 3 days of indirect co-culture with EPCs and the apoptosis rate was significanty reduced at 10 days of indirect co-culture with EPCs (both P < 0.05). These results suggest that EPCs can promote the proliferation but inhibit the apoptosis of OVX- BMSCs.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Osteoporosis, Postmenopausal, Endothelial Cells, Coculture Techniques, Cell Proliferation, Apoptosis, Tissue Engineering

Liu Zhu-ying, Chen Ying, Liu Qian, Liang Yuan, Zhang Rui, Wen Yi, Ding Yin. Indirect co-culture with endothelial progenitor cells improves proliferation and apoptosis of bone marrow mesenchymal stem cells of osteoporosis rats[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(14): 1999-2006.

Figures/Tables 11

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