Nano-hydroxyapatite effects on bone regeneration

doi:10.3969/j.issn.2095-4344.2015.08.002

Abstract

Abstract:

BACKGROUND: Hydroxyapatite materials have similar spatial structure and hardness to the natural bone matrix, but due to the imperfect early technology, there are some problems in the pore and degradation.

OBJECTIVE: To compare the effects of nano-hydroxyapatite materials and hydroxyapatite materials in the repair of disconnected defects of the tibia in rats.

METHODS: Thirty-six Sprague-Dawley rats were randomized into four groups, and a 5 mm disconnected defect model was established in the left tibia. Bone marrow mesenchymal stem cells/nano-hydroxyapatite complex was implanted into the experimental group, bone marrow mesenchymal stem cells/hydroxyapaptite complex into the control group, and bone marrow mesenchymal stem cells into the cell group. There was no treatment in the blank control group. At 2, 8, 12 weeks after implantation, X-ray measurement, hematoxylin-eosin staining, western blot assay were used for observation of bone repair, osteogenesis, and expression of type I collagen in the repair area.

RESULTS AND CONCLUSION: After 12 weeks of implantation, in the experimental group, the newborn calluses covered the whole defect region and formed visible bridge connections, the scaffold was degraded mostly, a great amount of osteoid tissues were visible to form the bone trabecula with lamellar bone structure, and there were a lot of type I collagens; in the control group, the number of calluses in the defect area was increased, poor integration was found at the broken end, bone sclerosis developed, the scaffold was not completely degraded, bone trabecula and lamellar bone formed, and a large amount of type I collagen was visible but lower than that in the experimental group; in the cell group and blank control group, no obvious bone regeneration was seen, and the expression of type I collagen was weak. These findings suggest that nano-hydroxyapatite is better than hydroxyapatite to promote bone regeneration.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: Nanoparticles, Hydroxyapatites, Stem Cells

CLC Number:

R318

Song Hua, Ren Xiang-qian, Wei Dong-xing. Nano-hydroxyapatite effects on bone regeneration[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(8): 1155-1159.

Figures/Tables 1

2.1 实验动物数量分析 36只SD大鼠均进入结果分析。 2.2 纳米羟基磷灰石支架材料内部结构特征纳米羟基磷灰石支架材料表面光滑，微粒结合紧密，彼此对齐，外观规则，其弹性、柔韧性及连续性较好，质地均匀。扫描电镜观察材料的内部结构发现，内部充满大小较为均一、球形的孔隙，孔隙直径为140-190 μm；孔与孔间还可见更小的孔隙互为连接贯通，其直径为10-35 μm，平均22 μm；孔壁为质地均匀的羟基磷灰石颗粒，紧密且连续(图1)。 2.3 术后骨缺损区形态学及组织学观察术后2周时：实验组、对照组支架网孔内可见大量细胞，细胞组、空白对照组未见有任何成骨样结构，有少量结缔组织。术后8周时：实验组、对照组移植物内开始有新生微血管生成，可看到成熟的骨组织向内部生长，同时出现骨髓结构和血管，支架材料已经部分降解，可见新生的类骨质形成，骨单位结构清晰，可见骨细胞发育良好，细胞排列紧密，并出现骨陷窝；细胞组、空白对照组缺损处除有结缔组织长入外，没有明显的成骨痕迹。术后12周时：实验组支架材料大部分已降解，可见大量的骨样组织并形成小梁，已经形成板层骨样结构(图2A)；对照组仍然有较多支架材料未降解，出现骨小梁样结构，亦有板层骨结构(图2B)；细胞组仅在边缘有少量骨板形成，可见大量结缔组织填充(图2C)；空白对照组缺损处几乎全部被纤维组织填充，缺损处仍无骨组织(图2D)。 2.4 术后骨缺损区X射线结果实验组术后2周时胫骨缺损处略见骨痂形成，呈薄云雾状；8周时缺损区两端可见明显的新生骨痂，12周时整个缺损区均可见新生骨痂且新骨密度增高，形成明显骨缺损区的桥接(图3A)。对照组术后2周时骨缺损区无明显变化，8周时仅在两断端有少量的云雾状骨痂形成，12周时骨缺损区骨痂形成增多，但仍未出现桥接，可见两端有骨质硬化现象(图3B)。细胞组、空白对照组骨缺损处未见骨性修复(图3C)。 2.5 免疫印记检测结果术后2周时，实验组、对照组可见Ⅰ型胶原蛋白表达，细胞组、空白对照几乎没有表达； 8周时，实验组、对照组Ⅰ型胶原蛋白表达明显增加，细胞组Ⅰ型胶原蛋白表达较弱，D组未检测到，Ⅰ型胶原蛋白表达量实验组> 对照组> 细胞组> 空白对照组，差异有显著性意义(P < 0.05)；12周时，实验组、对照组可见大量的Ⅰ型胶原蛋白阳性表达，细胞组、空白对照组Ⅰ型胶原蛋白表达较弱(图4)，各组间比较差异有显著性意义(P < 0.05)。"

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