Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (8): 1160-1164.doi: 10.3969/j.issn.2095-4344.2015.08.003

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Feasibility of nano-hydroxyapatite/chitosan/calcium sulphate hemihydrate as an injectable bone tissue engineering scaffold  

Xue Zhen1, Niu Li-yuan2, An Gang3, Guo Ya-shan3, Lv Song-cen1   

  1. 1The Fourth Ward, Department of Orthopedic Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China; 2Affiliated Hospital of Harbin Institute of Technology, Harbin 150086, Heilongjiang Province, China; 3Department of Emergency Trauma, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
  • Revised:2015-01-15 Online:2015-02-19 Published:2015-02-19
  • Contact: Xue Zhen, the Fourth Ward, Department of Orthopedic Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
  • About author:Xue Zhen, Master, Attending physician, the Fourth Ward, Department of Orthopedic Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
  • Supported by:

    the Science and Technology Research Project of Heilongjiang Provincial Education Department, No. 12511202

     

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Abstract:

BACKGROUND: In preliminary experiments, injectable nano-hydroxyapatite/chitosan/calcium sulphate hemihydrates (nHA/CS/CSH) composites have been developed based on the bionics principle, but the biocompatibility of this composite with bone marrow mesenchymal stem cells is not very clear.

OBJECTIVE: To study the feasibility of nHA/CS/CSH as an injectable bone tissue engineering scaffold.
METHODS: Bone marrow mesenchymal stem cells from rabbits were cultured and induced, and the third passage cells were cultured with nHA/CS/CSH as an experimental group. Bone marrow mesenchymal stem cells subjected to inoculated culture were used as controls. The morphology and growth of the cells were observed under inverted phase contrast microscope. MTT assay was used to examine cell proliferation. The morphology and proliferation of the cells on the surface of nHA/CS/CSH was observed by scanning electron microscope. The nHA/CS/CSH scaffold was implanted into the rabbit sacrospinal muscles, and pathologically observed after 2, 4, 6, 8 weeks.

RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells co-cultured with nHA/CS/CSH exhibited good growth as observed under inverted phase contrast microscope, without significant difference from those in the control group. At 2 weeks after scaffold implantation, a moderate amount of neutrophils, lymphocytes and giant cells infiltrated around the graft material, small blood vessels and fibroblasts proliferated, and the graft material was divided and embraced by inflammatory cells; at 4 weeks after implantation, a few lymphocytes, fibroblasts aggregated, the inflammation subsided further, and muscle fibers showed normal arrangement and morphology; at 6 weeks after implantation, the inflammation around the graft material became mild and tissue edema was not obvious; at 8 weeks after implantation, the inflammation subsided basically, the material degradation was complete, and the muscle fibers had normal morphology. These findings indicate that the nHA/CS/CSH composite material has good biocompatibility and biodegradation, which may be used as a good scaffold material for injectable bone tissue engineering scaffold.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: Tissue Engineering, Hydroxyapatites, Chitosan

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