Neural stem cell transplantation for partial sciatic nerve transaction-induced neuropathic pain: the optimal cell number for transplantation

doi:10.3969/j.issn.2095-4344.2015.45.018

Abstract

Abstract:

BACKGROUND: Studies have shown that neural stem cell transplantation has a certain effect on neuropathic pain, but the efficacy of transplanted cell number on neuropathic pain is not exactly understood.
OBJECTIVE: To observe the effect of different amount of neural stem cells administered intrathecally on the neuropathic pain and expression of glial-derived neurotrophic factor in the spinal cord dorsal horn and dorsal root ganglion in rats after partial sciatic nerve transaction.
METHODS: A Sprague-Dawley rat at 14-16 days of pregnancy was used to prepare neural stem cell suspensions that were injected intrathecally into rat models of partial sciatic nerve transaction at doses of 1×103, 1×104, 1×105, 1×106, 1×107 cells per 30 μL, respectively. Additionally, model group and sham-operated group were set up. Threshold values of mechanical and thermal pain were recorded 1 day before operation, 1, 3, 7, 14,
21 days after operation. Expressions of glial-derived neurotrophic factor protein and mRNA in the ipsilateral spinal cord dorsal horn and dorsal root ganglion were detected by immunohistochemistry and RT-PCR, respectively, at 7 and 21 days after partial sciatic nerve transaction.
RESULTS AND CONCLUSION: Pain threshold values were decreased in all the groups except the sham-operated group at 1 day after operation, and reached the peak at 7 and 14 days after operation (P < 0.05). Compared with the model group, the pain threshold values and the expression of glial-derived neurotrophic factor protein and mRNA in the ipsilateral spinal cord dorsal horn and dorsal root ganglion were increased gradually in a dose-dependent manner in the 1×104, 1×105, 1×106, 1×107 groups at 7 days after operation (P < 0.05). At 21 days post-operation, the pain threshold values showed no differences from the preoperative findings in the 1×105, 1×106, 1×107 groups, but the expression of glial-derived neurotrophic factor was significantly higher in the 1×105 group than the other groups (P < 0.05). Taken together, intrathecal transplantation of neural stem cells at a dose of 1×105 is the most effective in alleviating partial sciatic nerve transaction-induced neuropathic pain in rats.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Cell Transplantation, Sciatic Nerve, Pain, Neurturin, Tissue Engineering

Deng Mo, Zhao Feng. Neural stem cell transplantation for partial sciatic nerve transaction-induced neuropathic pain: the optimal cell number for transplantation[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(45): 7314-7319.

Figures/Tables 5

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