Calcium alginate/bone marrow mesenchymal stem cells combined with degradation membrane for repair of skin defects

doi:10.3969/j.issn.2095-4344.2015.32.022

Abstract

Abstract:

BACKGROUND: Skin transplantation is regarded as the most effective therapy for large-area skin defects, which is limited by donor sources and immune rejection. Therefore, it is extremely accelerate the construction of the dermis in skin tissue engineering.
OBJECTIVE: To investigate the effects of bone marrow mesenchymal stem cells/calcium alginate gel, basic fibroblast growth factor and degradation membrane on the repair of full-thickness skin defects.
METHODS: Bone marrow mesenchymal stem cells were isolated from 15 New Zealand rabbits, and then cultured, amplified, subcultured and purified. Three full-thickness skin defects were made on the back of every rabbit, and randomly treated with bone marrow mesenchymal stem cells/calcium alginate gel, basic fibroblast growth factor and degradation membrane as experimental group, bone marrow mesenchymal stem cells/calcium alginate gel as control 1 group, and calcium alginate gel as control 2 group. The wounds were all covered with amniotic membrane. After 7, 14, 21 days, new wound tissues were taken for hematoxylin-eosin staining, immunohistochemistry staining and image analysis.
RESULTS AND CONCLUSION: Dermis tissues in the experimental group were obviously thicker than those in control 1 and control 2 groups; there were more fibroblasts, vessels and collagen fibers in the experimental group. Especially at 14 and 21 days after operation, epidermal hyperplasia was faster with a larger coverage area in the experimental group, and at 21 days, the new epidermal tissues mainly exhibited multi-layered structure, which was superior to the control 1 and 2 groups. It follows that the combination of bone marrow mesenchymal stem cells/calcium alginate gel, basic fibroblast growth factor and degradation membrane for skin defects can accelerate the repair and regeneration of the dermis, and thus promote the epidermis regeneration and reconstruction.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Alginates, Fibroblast Growth Factor 2

CLC Number:

R394.2

Wang Nan, Sun Pei-jie, Su Qiu-xiang, Fu Ling, Zhou Zhe, Li Na-ran, Yang Xiao-xia. Calcium alginate/bone marrow mesenchymal stem cells combined with degradation membrane for repair of skin defects[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(32): 5204-5209.

Figures/Tables 5

References

[1] Tufaro AP, Buck DW 2nd, Fischer AC. The use of artificial dermis in the reconstruction of oncologic surgical defects. Plast Reconstr Surg. 2007;120(3):638-646.

[2] Ehrlich HP. Understanding experimental biology of skin equivalent: from laboratory to clinical use in patients with burns and chronic wounds. Am J Surg. 2004;187(5A):29S-33S.
[3] 中华人民共和国科学技术部. 关于善待实验动物的指导性意见.2006-09-30.
[4] Watt FM. Epidermal stem cells as targets for gene transfer. Hum Gene Ther. 2000;11(16):2261-2266.
[5] Nugent MA, Iozzo RV. Fibroblast growth factor-2. Int J Biochem Cell Biol. 2000;32(2):115-120.
[6] Li A, Simmons PJ, Kaur P. Identification and isolation of candidate human keratinocyte stem cells based on cell surface phenotype. Proc Natl Acad Sci U S A. 1998;95(7): 3902-3907.
[7] Rydén M, Dicker A, Götherström C, et al. Functional characterization of human mesenchymal stem cell-derived adipocytes. Biochem Biophys Res Commun. 2003;311(2): 391-397.
[8] Dominici M, Le Blanc K, Mueller I, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006;8(4):315-317.
[9] Lu D, Mahmood A, Wang L, et al. Adult bone marrow stromal cells administered intravenously to rats after traumatic brain injury migrate into brain and improve neurological outcome. Neuroreport. 2001;12(3):559-563.
[10] Badiavas EV, Falanga V. Treatment of chronic wounds with bone marrow-derived cells. Arch Dermatol. 2003;139(4): 510-516.
[11] Rasulov MF, Vasilchenkov AV, Onishchenko NA, et al. First experience of the use bone marrow mesenchymal stem cells for the treatment of a patient with deep skin burns. Bull Exp Biol Med. 2005;139(1):141-144.
[12] 方利君,付小兵,王玉新,等.体外诱导骨髓间充质干细胞分化为上皮细胞的研究[J].中华实验外科杂志,2004,21(20):171-173.
[13] 李文辉,侯筱魁,汤亭亭,等.复合骨髓基质细胞的藻酸钙凝珠体外培养特征[J].中国临床康复,2005,9(30):260-262.
[14] 何志伟,赵建宁.藻酸钙复合材料在软骨组织工程中的应用前景[J].中国骨伤,2007,20(11):789-791.
[15] 沙德峰,辛畅泰,杨晓霞,等. 肌腱损伤缝接处置入碱性成纤维细胞生长因子复合缓释降解膜的研究[J].中国修复重建外科杂志, 2004,18(2):148-151.
[16] 杨晓霞,辛畅泰,孙佩杰,等.碱性成纤维细胞生长因子复合降解膜置入脊髓缺损局部对脊髓神经再生的作用[J].中国临床康复, 2004, 8(20):4002-4003.
[17] Pierce GF, Tarpley JE, Yanagihara D,et al.Platelet-derived growth factor (BB homodimer), transforming growth factor-beta 1, and basic fibroblast growth factor in dermal wound healing. Neovessel and matrix formation and cessation of repair.Am J Pathol. 1992;140(6):1375-1388.
[18] 付小兵,王德文.创伤修复基础[M].北京:人民军医出版社.1997: 202-232.