EdU-labeled human umbilical cord mesenchymal stem cells in vitro: 5 and 10 µmol/L are the optimal concentrations of EdU

doi:10.3969/j.issn.2095-4344.2015.32.015

Abstract

Abstract:

BACKGROUND: EdU is a new nuclear marker, and currently, it is rarely reported.
OBJECTIVE: To determine the optimal concentration of EdU to label human umbilical cord mesenchymal stem cells.
METHODS: Human umbilical cord mesenchymal stem cells were isolated, purified and subcultured. Cell morphology and growth were observed under inverted microscope. Flow cytometry was used to identify cell surface markers, as well as adipogenic identification. EdU at concentrations of 5, 10, 20, 50, 100 µmol/L was used to label human umbilical cord mesenchymal stem cells for 24 hours. The optimal concentration that resulted in the highest labeling efficiency was selected, and then cell proliferation curve was drawn.
RESULTS AND CONCLUSION: Under the inverted microscope, cells grew adherently in a long spindle shape, and EdU-labeled cells had the same morphology. Flow cytometry showed that cells were positive for CD44, and had adipogenic differentiation ability. When the concentration of EdU was 5 and 10 µmol/L, the labeling efficiency was the highest, indicating that 5 and 10 µmol/L are the optimal concentrations of EdU to label human umbilical cord mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Umbilical Cord, Mesenchymal Stem Cells, Pyrimidine Nucleosides

CLC Number:

R394.2

Wang Wu, Zhang Fei, Li Gui-cai, Qi Bao-jun, Wu Zhong-yan, Cao Peng. EdU-labeled human umbilical cord mesenchymal stem cells in vitro: 5 and 10 µmol/L are the optimal concentrations of EdU [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(32): 5167-5171.

Figures/Tables 3

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