Establishing an animal model of delayed onset muscle soreness and its histomorphologic observation

doi:10.3969/j.issn.2095-4344.2015.29.011

Abstract

Abstract:

BACKGROUND: Delayed onset muscle soreness is closely related to skeletal muscle micro-injury, but the exact mechanism of skeletal muscle micro-injury is not yet clear.
OBJECTIVE: To observe the histomorphological and ultrastructure changes of skeletal muscle micro-injury models induced by eccentric exercise.
METHODS: Forty male Sprague-Dawley rats were randomly divided into quiet control group, immediately after exercise group, post-exercise 24 hours group, post-exercise 48 hours group and post-exercise 72 hours group. In the latter four groups, the rats were subjected to intermittent running on the -16° slope at a speed of 16 m/min: 5 minutes movement, 2 minutes rest and totally 120 minutes. Rats in the latter four groups were observed immediately, at 24, 48, 72 hours after exercise.
RESULTS AND CONCLUSION: After eccentric exercise, the morphology and ultrastructure of rat’s skeletal muscle were both changed to different extents, and Desmin and Vimentin were dyed off for anti-desmin antibody staining at varying degrees. It indicates that one-time eccentric exercise can cause delayed skeletal muscle micro-injury.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Sports, Muscle, Skeletal, Desmin, Vimentin

CLC Number:

R318

Wei Yuan, Fang Chun-lu, Li Liang-ming, Xing Wen-hua, Yang Ze-yi. Establishing an animal model of delayed onset muscle soreness and its histomorphologic observation[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(29): 4645-4651.

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