Umbilical cord blood mesenchymal stem cell transplantation for Parkinson’s disease: a feasibility study

doi:10.3969/j.issn.2095-4344.2015.28.025

Abstract

Abstract:

BACKGROUND: Stem cells can be induced to differentiate into dopaminergic neurons in vivo and in vitro, which provides a theoretical basis for stem cell transplantation in the treatment of Parkinson’s disease
OBJECTIVE: To explore the feasibility and mechanism of intracerebral transplantation of umbilical cord blood mesenchymal stem cells for treatment of Parkinson’s disease rats.
METHODS: Intracerebral injection of 6-hydroxydopamine was used to make Parkinson’s disease models in SD rats. Twenty-two model rats were randomized into cell transplantation group (n=12) and control group (n=10) and respectively injected intracerebrally with umbilical cord blood mesenchymal stem cell suspension and PBS. At 1-8 weeks after cell transplantation, intra-abdominal injection of apomorphine was performed every week to observe the rotation behaviors of rats; at the 2nd and 8th weeks, rat’s striatum and substantia nigra were taken for immunohistochemistry staining.
RESULTS AND CONCLUSION: The rotation behaviors were gradually decreased with time in the cell transplantation, but had no changes in the control group. At 3-8 weeks after transplantation, there were significant differences in the rotation behaviors between the two groups (P < 0.05). At 2 weeks after transplantation, tyrosine hydroxylase-positive cells were found within and around the striatum of the cell transplantation group; but there were no exogenous cells in the control group. At 8 weeks after transplantation,
there were still active cells and tyrosine hydroxylase-positive cells in the striatum of cell transplantation group, and there was no tyrosine hydroxylase expression in the striatum of the control group. These findings suggest that transplanted umbilical cord blood mesenchymal stem cells can survive in the brain that are positive for tyrosine hydroxylase, which can improve the behavior abnormalities of Parkinson’s disease rats.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Cord Blood Stem Cell Transplantation, Parkinson Disease, Hydroxydopamines, Rotation

CLC Number:

R394.2

Liu Lei, Feng De-peng, Chen Yan, Zhao Xiu-min, Feng Xiao-ya, Ge Ru-cun, Xun Ying, Lv Yong-tao . Umbilical cord blood mesenchymal stem cell transplantation for Parkinson’s disease: a feasibility study[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(28): 4567-4571.

Figures/Tables 2

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