Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (16): 2534-2539.doi: 10.3969/j.issn.2095-4344.2015.16.014

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Nano-hydroxyapatite is non-toxic to human umbilical cord vein endothelial cells

Cheng Guang-cun, Yan Zhong-ya, Li Chun-sheng, Yan Yu, Wei Xiao-yong   

  1. Department of Cardiothoracic Surgery, the Affiliated Provincial Hospital of Anhui Medical University, Anhui Provincial Institute of Cardiovascular Disease, Hefei 230001, Anhui Province, China
  • Received:2015-03-01 Online:2015-04-16 Published:2015-04-16
  • Contact: Yan Zhong-ya, Professor, Chief physician, Department of Cardiothoracic Surgery, the Affiliated Provincial Hospital of Anhui Medical University, Anhui Provincial Institute of Cardiovascular Disease, Hefei 230001, Anhui Province, China
  • About author:Cheng Guang-cun, Master, Associate chief physician, Associate professor, Department of Cardiothoracic Surgery, the Affiliated Provincial Hospital of Anhui Medical University, Anhui Provincial Institute of Cardiovascular Disease, Hefei 230001, Anhui Province, China
  • Supported by:

    the Natural Science Foundation of Anhui Province, No. 1308085MH143

Abstract:

BACKGROUND: Pulsed laser deposition synthesis technology has been used to prepare new nano-hydroxyapatite thin film coating by collagen deposition on artificial mechanical heart valve. 
OBJECTIVE: To investigate the toxicity of new nano-hydroxyapatite thin film on human umbilical vein endothelial cells.
METHODS: Human umbilical vein endothelial cells were cultured with nano-hydroxyapatite film room-temperature leaching solution, nano-hydroxyapatite film high-temperature leaching solution, high-density polyethylene and phenol solution. Within 72 hours, cell growth was observed under the inverted phase contrast microscope. At 7 days after culture, cell proliferation and toxicity grading were detected using Cell Counting Kit-8. 
RESULTS AND CONCLUSION: At 24 hours after culture, cells grew well, showed fusiform shape, and had strong refraction in the nano-hydroxyapatite film room-temperature leaching solution, nano-hydroxyapatite film high-temperature leaching solution, high-density polyethylene groups, and no significant differences in cell morphology and number were detected among above groups. Cells in the phenol solution group were suspended, round, pyknotic and dead. At 48 hours, except phenol solution group, cell number increased significantly, and cell grew densely in other three groups. At 72 hours, cells grew strongly, and the gap became small obviously. Within 7 days after culture, cell proliferation activity was not significant in the nano-hydroxyapatite film room-temperature leaching solution, nano-hydroxyapatite film high-temperature leaching solution, and high-density polyethylene groups, which was significantly higher than in the phenol solution group (P < 0.05). The toxicity of nano-hydroxyapatite film graded 0 to 1. These results suggested that nano-hydroxyapatite artificial mechanical heart valve has good histocompatibility, but no toxicity.


中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


全文链接:

Key words: Durapatite, Endothelial Cells, Umbilical Veins

CLC Number: