Effect of lipopolysaccharide stimulation combined with heat stress on release of interleukin-6 and tumor necrosis factor-alpha from human intestinal epithelial cells cultured in vitro

doi:10.3969/j.issn.2095-4344.2014.51.008

Abstract

Abstract:

BACKGROUND: There is no practical and reliable experimental evidence for changes in molecular biology of intestinal epithelial cells suffering from heat stress, intestinal bacteria and endotoxin produced by bacteria.

OBJECTIVE: To observe the effect of lipopolysaccharide stimulation combined with heat stress on human intestinal epithelial cell injury and release of interleukin-6 and tumor necrosis factor-alpha.

METHODS: Human intestinal epithelial cell lines (SW 480) cultured in vitro was seeded into culture dishes at a density of 3×10⁵, and then divided randomly into four groups: control group was cultured in an incubator of 37 ℃; heat stress group was cultured in the incubator of 43 ℃ for 2 hours; lipopolysaccharide group was stimulated by 1 mg/L lipopolysaccharide for 6 hours; lipopolysaccharide+heat stress group (combination group) was subjected to heat stress for 2 hours followed by lipopolysaccharide stimulation for 6 hours. WST-1 method was used to investigate the cytotoxic effect and cell survival rate, and ELISA assay was used to investigate the levels of interleukin-6 and tumor necrosis factor-alpha.

RESULTS AND CONCLUSION: Compared with the control group, the cytotoxic effect increased, and the cell survival rate reduced as well as the level of interleukin-6 and tumor necrosis factor-alpha increased in the heat stress and lipopolysaccharide groups. Synergistic effects were visible in the combination group. These findings indicate that lipopolysaccharide and heat stress alone can both induce cell injury, increase the levels of interleukin-6 and tumor necrosis factor-alpha, and there is a synergistic effect between them. Experimental findings from this study may be an objective experimental basis for understanding the pathogenesis of multiple organ failure caused by bowel dysfunction, intestinal bacteria and endotoxin translocation during the pathological process of severe heat stroke.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: lipopolysaccharides, epithelial cells, interleukin-6, tumor necrosis factor-alpha

CLC Number:

R318

Sun Jie, Chen Rui, Dong Jia-hui, Jiang Qiao, Sun Zhao-kun, Guo Zhen-hui. Effect of lipopolysaccharide stimulation combined with heat stress on release of interleukin-6 and tumor necrosis factor-alpha from human intestinal epithelial cells cultured in vitro[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(51): 8238-8242.

Figures/Tables 3

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