Polylactic-co-glycolic acid complex with different concentrations of Schwann cells for peripheral nerve regeneration

doi:10.3969/j.issn.2095-4344.2014.47.007

Abstract

Abstract:

BACKGROUND: Studies have shown that nerve grafts with Schwann cells can repair peripheral nerve defect and Schwann cells have an important role in nerve regeneration.
OBJECTIVE: To observe the rehabilitation status of neutral function after sciatic nerve injury in rats bridged by nerve grafts with Schwann cells.
METHODS: A rat model of sciatic nerve injury was established, and schwann cells were primarily cultured. Then, the rat model was repaired with polylactic-co-glycolic acid copolymer-extracellular matrix gel-Schwann cells complex. According to different concentrations of Schwann cells, there were five cell groups from 10⁵/L to 10⁹/L and a control group. The nerve conduction velocity was detected respectively at 3, 6, 12 weeks after modeling; the e tibialis anterior muscle gravity was detected and histological observation was done at 12 weeks.
RESULTS AND CONCLUSION: The nerve conduction velocities in the cell groups were all higher than that in the control group at 3, 6, 12 weeks after modeling (P < 0.01), and it was highest in the 10⁸/L (P < 0.05). At 12 weeks, hematoxylin-eosin staining of the tibialis anterior muscle showed that the number of normal muscle fibers was higher in the cell groups than the control group (P < 0.05). In the 10⁸/L and 10⁹/L groups, the morphology of tibialis anterior muscle recovered well; the muscle fibers were in strip-like and wavy shapes, grew in the same direction, and had similar length, thickness and density. These findings indicate that polylactic-co-glycolic acid complex with 10⁸/L Schwann cells is better to promote sciatic nerve regeneration.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: peripheral nerves, Schwann cells, sciatic nerve

CLC Number:

R318

Sun Huan-wei, Zhang Tie-hui, You Xin-yan, Ren Yuan-fei, Zhong Sheng. Polylactic-co-glycolic acid complex with different concentrations of Schwann cells for peripheral nerve regeneration[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(47): 7579-7584.

Figures/Tables 2

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