Human nerve growth factor beta-modified bone marrow mesenchymal stem cells from the rabbit mandible by transfection of lentiviral vectors

doi:10.3969/j.issn.2095-4344.2014.37.002

Abstract

Abstract:

BACKGROUND: Central nerve damage and peripheral nerve injury are common clinical problems that have no ideal treatment. Nerve growth factor has an important role in neuronal repairing and growth. But its local injections may have shorts of inactivation and loss.
OBJECTIVE: To construct human nerve growth factor beta recombinant plasmids, which are transfected into bone marrow mesenchymal stem cells from the rabbit mandible by lentiviral vectors, and to investigate the bioactivity of human nerve growth factor beta.
METHODS: pDC316-hNGFβ-mCMV-EGFP plasmids were constructed via lentiviral vectors using Hind III+Not I digestion. Bone marrow mesenchymal stem cells from the rabbit mandible were isolated and cultured, and then transfected by recombinant plasmids. The expression of human nerve growth factor beta in transfected cells was detected by ELISA method.
RESULTS AND CONCLUSION: pDC316-hNGFβ-mCMV-EGFP plasmids were proved to be constructed successfully by gene sequencing and enzyme identification. The transfected cells under a fluorescence microscope emitted green fluorescence, and the fluorescence intensity had no change with incubation time. The expression of human nerve growth factor beta was maintained at a level of 25 μg/L at 7 days after cell transfection, and the bioacitivty was increased significnalty.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cells, mandible, nerve growth factor, lentivirus infections

CLC Number:

R394.2

Liu Xiao-chang, Zhao Ying-hua, Yang Zi-gui, Wang Lei . Human nerve growth factor beta-modified bone marrow mesenchymal stem cells from the rabbit mandible by transfection of lentiviral vectors[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(37): 5911-5915.

Figures/Tables 1

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