Effect of different concentrations of human amniotic homogenate supernatant on the proliferation of rat Schwann cells

doi:10.3969/j.issn.2095-4344.2014.20.019

Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (20): 3218-3222.doi: 10.3969/j.issn.2095-4344.2014.20.019

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Effect of different concentrations of human amniotic homogenate supernatant on the proliferation of rat Schwann cells

Liu Liang¹, Wang Lei¹, Tong Ya-lin², Mo Yong-liang¹, Lv Lu¹, Chen Yun-peng¹, Yang Wen-xian³, Lv Li-fang³, Zhan Qiu³, Zhu Fu-jun², Xin Hai-ming², Gong Zhen-yu²

1Department of Burn and Plastic Surgery, the 181 Hospital of Chinese PLA, Postgraduate Cultivation Basement of Guangxi Normal University, Guilin 541002, Guangxi Zhuang Autonomous Region, China; 2Department of Burn and Plastic Surgery, the 181 Hospital of Chinese PLA, Guilin 541002, Guangxi Zhuang Autonomous Region, China; 3Experimental Research Center, the 181 Hospital of Chinese PLA, Central Laboratory of Burn and Plastic Surgery, Guangzhou Military Region of Chinese PLA, Guilin 541002, Guangxi Zhuang Autonomous Region, China

Received:2014-04-03 Online:2014-05-14 Published:2014-05-14
Contact: Tong Ya-lin, Chief physician, Department of Burn and Plastic Surgery, the 181 Hospital of Chinese PLA, Guilin 541002, Guangxi Zhuang Autonomous Region, China
About author:Liu Liang, Studying for master’s degree, Department of Burn and Plastic Surgery, the 181 Hospital of Chinese PLA, Postgraduate Cultivation Basement of Guangxi Normal University, Guilin 541002, Guangxi Zhuang Autonomous Region, China
Supported by:
Science Research and Technology Development Project of Guangxi Zhuang Autonomous Region, No. 1140003A-39; Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 0991290, 2011GXNSFB018107; National Natural Science Foundation of China for Youths, No. 81301634; Military Medical Science Research Project During the Twelfth Five-Year Plan, No. CWS11J277

Abstract

Abstract:

BACKGROUND: Schwann cells are important cell lines in the process of repairing peripheral nerve injury, and human amniotic homogenate supernatant is shown to secrete a variety of cytokines, which could promote the proliferation of Schwann cells.
OBJECTIVE: To investigate the effect of different concentrations of human amniotic homogenate supernatant on the proliferation of rat Schwann cell 96.
METHODS: Schwann cell 96 was cultured with high-glucose DMEM containing 20% fetal bovine serum, and the second generation of Schwann cell 96 was applied for experiments. The cultured cells were divided into five groups according to different volume fractions of human amniotic homogenate supernatant (0%, 10%, 15%, 20%, 25%) in the medium.
RESULTS AND CONCLUSION: The total protein concentration of human amniotic homogenate supernatant was
675 μg/mL, in which the concentration of epidermal growth factor, basic fibroblast growth factor and vascular endothelial growth factor were respectively (470.625±2.546), (4.121±0.026) and (0.172±0.002) ng/L. At 1-7 days, the cell proliferation rate of the 10% and 15% concentration groups was greater than that in 20% and 25% concentration groups (P < 0.05); 10% and 15% concentrations promoted cell proliferation, while 20% and 25% concentrations inhibited cell proliferation. There were no significant difference in the viability of Schwann cell 96 between the control group and the experimental group (P > 0.05). Low concentrations (10%, 15%) of human amniotic homogenate supernatant promote the proliferation of Schwann cell 96, while high concentrations (20%, 25%) of human amniotic homogenate supernatant inhibit cell proliferation.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: amnion, Schwann cells, cell proliferation, cytokines

CLC Number:

R318

Liu Liang, Wang Lei, Tong Ya-lin, Mo Yong-liang, Lv Lu, Chen Yun-peng, Yang Wen-xian, Lv Li-fang, Zhan Qiu, Zhu Fu-jun, Xin Hai-ming, Gong Zhen-yu . Effect of different concentrations of human amniotic homogenate supernatant on the proliferation of rat Schwann cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(20): 3218-3222.

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Effect of different concentrations of human amniotic homogenate supernatant on the proliferation of rat Schwann cells

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