Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (11): 1965-1971.doi: 10.3969/j.issn.2095-4344.2013.11.010
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Sun Hong1, Zhang Ming-hui2, Weng Li-xin3, Sun Tong-zhu4
Received:
2012-06-06
Revised:
2012-07-06
Online:
2013-03-12
Published:
2013-03-12
Contact:
Weng Li-xin, Associate professor, Master’s supervisor, Department of Pathology, Basic Medical School of Inner Mongolia Medical University, Hohhot 010059, Inner Mongolia Autonomous Region, China
wenglixin2007@yahoo.cn
About author:
Sun Hong★, Master, Basic Medical School of Inner Mongolia Medical University, Hohhot 010059, Inner Mongolia Autonomous Region, China
wenglixin2007@yahoo.cn
Zhang Ming-hui, Master, Gansu University of Traditional Chinese Medicine, Lanzhou 730000, Gansu Province, China
hhhtzmh@163.com
Sun Hong and Zhang Ming-hui contributed equally to this work.
CLC Number:
Sun Hong, Zhang Ming-hui, Weng Li-xin, Sun Tong-zhu. Acidic fibroblast growth factor promotes the repair of intestinal ischemia-reperfusion injuries in rats[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(11): 1965-1971.
2.1 实验动物数量分析 实验共纳入110只大鼠,造模过程部分死亡,72只大鼠进入结果分析,造模成功率65.5%。实验过程中,模型组和治疗组均有死亡,原因是低血容量休克,均剔除实验,最后每组各取8只进入结果分析。 2.2 小肠组织的病理学改变 假手术组小肠黏膜上皮及腺体完好,未见明显的炎细胞浸润,见图1。小肠缺血-再灌注后早期病变较轻,表现为黏膜和黏膜下充血,固有层多量中性粒细胞浸润,可见黏膜上皮变性、坏死、脱落,再灌注后2,6,12 h较为明显,在再灌注后24 h充血明显减轻,上皮细胞开始再生,见图2。经酸性成纤维细胞生长因子治疗后,小肠黏膜充血、炎细胞浸润及上皮糜烂坏死的程度在各时间点较模型组显著改善,见图3。模型组再灌注24 h的黏膜表现与治疗组再灌注12 h相似。"
2.5 小肠组织细胞外调节蛋白激酶的表达情况 假手术组和模型组:在正常和伤后大鼠,绒毛上皮、小肠隐窝和固有层均可见到染色阳性的细胞。在假手术组大鼠的绒毛上皮和小肠隐窝,阳性细胞的比例分别为59%和27.5%,表现为散在分布于胞浆内的棕色颗粒,大部分靠近细胞核,少量细胞有核内表达。小肠隐窝核内表达细胞外调节蛋白激酶的细胞主要位于中下部,相当于干细胞、短暂扩增细胞及其初级子代细胞的位置。杯状细胞的胞浆内也可见阳性颗粒。模型组几乎全部绒毛上皮细胞和隐窝细胞表达磷酸化细胞外调节蛋白激酶,但随着再灌注时间的延长,核内表达的比例增加。模型组2 h和6 h时阳性颗粒主要位于胞浆中,12 h则主要位于细胞核,核内表达的比例下降接近假手术组。各组动物的黏膜固有层均有少量的细胞,正常情况下主要位于胞浆,随着再灌注时间延长核内表达增多,在模型组12 h最显著。见表3,图6。"
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