Acidic fibroblast growth factor promotes the repair of intestinal ischemia-reperfusion injuries in rats

doi:10.3969/j.issn.2095-4344.2013.11.010

Abstract

Abstract:

BACKGROUND: Role of extracellular signal-regulated kinase and acidic fibroblast growth factor receptor 2 in intestinal ischemia-reperfusion injury repair has not been reported.
OBJECTIVE: To identify the changes in extracellular signal-regulated kinase and acidic fibroblast growth factor receptor 2 regulated by exogenous acidic fibroblast growth factor (acidic fibroblast growth factor) following intestinal ischemia-reperfusion injury in rats, and to explore the role of extracellular signal-regulated kinase and acidic fibroblast growth factor receptor 2 in acidic fibroblast growth factor-induced injury repair.
METHODS: Intestinal ischemia-reperfusion injury models were produced in rats by clamping the superior mesenteric artery for 45 minutes, and then acidic fibroblast growth factor administration was applied immediately after modeling. Tissue specimens were collected at 2, 6, 12, and 24 hours after reperfusion. The expressions of acidic fibroblast growth factor receptor 2 and extracellular signal-regulated kinase were detected by immunohistochemistry and reverse transcription-PCR.
RESULTS AND CONCLUSION: The expressions of acidic fibroblast growth factor receptor 2 and extracellular signal-regulated kinase increased gradually after ischemia-reperfusion injury and peaked at 6-12 hours after reperfusion. Acidic fibroblast growth factor administration relieved intestinal mucosa injury and increased the expressions of acidic fibroblast growth factor receptor 2 and extracellular signal-regulated kinase. These results suggest that acidic fibroblast growth factor administration can upregulate the expressions of acidic fibroblast growth factor receptor 2 and extracellular signal-regulated kinase after ischemia-reperfusion injury, indicating exogenous acidic fibroblast growth factor can participate in visceral damage repair via the activation of endogenous acidic fibroblast growth factor receptor 2 and extracellular signal-regulated kinase.

Key words: tissue construction, tissue construction and bioactive factor, acidic fibroblast growth factor, receptor, extracellular signal-regulated kinase, ischemia-reperfusion injury, small intestine, intestinal villus epithelial cells, other grants-supported paper, tissue construction photographs-containing paper

CLC Number:

R318

Sun Hong, Zhang Ming-hui, Weng Li-xin, Sun Tong-zhu. Acidic fibroblast growth factor promotes the repair of intestinal ischemia-reperfusion injuries in rats[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(11): 1965-1971.

Figures/Tables 10

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