Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (48): 9039-9043.doi: 10.3969/j.issn.2095-4344.2012.48.021

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Distribution and co-expression of neuropeptide Y/calcitonin gene-related peptide in the lumbar intervertebral disc

Wang Yong-gang, Zhou Hai-yu, Wang Shuan-ke, Wang Dong-min, Shi Pei-sheng, Wang Jing, Wang Cui-fang, Ma Jing-lin   

  1. Department of Orthopedics, Second Hospital of Lanzhou University, Key Laboratory of Bone and Joint Disease in Gansu Province, Lanzhou 730030, Gansu Province, China
  • Received:2012-02-22 Revised:2012-05-24 Online:2012-11-25 Published:2013-03-14
  • Contact: Zhou Hai-yu, Associate professor, Associate chief physician, Department of Orthopedics, Second Hospital of Lanzhou University, Key Laboratory of Bone and Joint Disease in Gansu Province, Lanzhou 730030, Gansu Province, China zhouhy@lzu.edu.cn
  • About author:Wang Yong-gang, Physician, Department of Orthopedics, Second Hospital of Lanzhou University, Key Laboratory of Bone and Joint Disease in Gansu Province, Lanzhou 730030, Gansu Province, China 13462717@qq.com

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Abstract:

BACKGROUND: Studies have found the co-existence of neuropeptide Y/calcitonin gene-related peptide in the sympathetic ganglia.
OBJECTIVE: To study the distribution of neuropeptide Y/calcitonin gene-related peptide in the normal lumbar disc and the co-expression in herniated lumbar nucleus pulposus tissue.
METHODS: Complete lumbar discs were collected from 10 cases of cadaver, and herniated lumbar nucleus pulposus tissues were collected from other 10 cases of cadaver as control. Thirty patients with the symptoms lumbar disc herniation were collected, lumbar nucleus pulposus of L4/5 and L5/S1 were removed and the nucleus pulposus tissues were obtained as control group.
RESULTS AND CONCLUSION: ①Positive neuropeptide Y/calcitonin gene-related peptide immunofluorescence double staining of nerve fibers were distributed in the 1/3 outside annulus fibrosus and few in 2/3 inside annulus fibrosus and nucleus pulposus in the normal lumbar intervertebral disc tissue. ②Co-expression of nucleus pulposus and the positive rate of neuropeptide Y/calcitonin gene-related peptide immunofluorescence double staining of nerve fibers in the experimental group were higher than those in the control group (P < 0.05). This study demonstrates that neuropeptide Y/calcitonin gene-related peptide distributes on the 1/3 outside annulus fibrosus in normal lumbar intervertebral disc, but do not distribute on the 2/3 inside of annulus fibrosus and nucleus pulposus. But a large number of neuropeptide Y/calcitonin gene-related peptide co-expressed in the herniated nucleus pulposus.

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