Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (48): 9044-9049.doi: 10.3969/j.issn.2095-4344.2012.48.022

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Expression of bone morphogenetic protein-2 and decorin at the fracture ends and the surrounding tissues of bone nonunion

Han Xin-guang1, Wang Dong-kui2, Gao Feng3, Liu Ren-hao1, Bi Zheng-gang1   

  1. 1Department of Orthopedics, 2Department of Radiology, 3Pharmacy Intravenous Admixture Service, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
  • Received:2012-02-07 Revised:2012-04-18 Online:2012-11-25 Published:2013-03-14
  • Contact: Bi Zheng-gang, Professor, Doctoral supervisor, Department of Orthopedics, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China bizhenggang@54dr.com
  • About author:Han Xin-guang☆, Doctor, Attending physician, Department of Orthopedics, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China hxg9908@163.com

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Abstract:

BACKGROUND: Bone morphogenetic protein-2 and decorin have the potential to promote fracture healing, and many reports have proved that they can promote each other in promoting fracture healing.
OBJECTIVE: To detect the expression of bone morphogenetic protein-2 and decorin at the fracture ends and the surrounding tissues of bone nonunion.
METHODS: Eleven patients with a delayed union (eight patients) and a nonunion (fifteen patients) were included. The average duration of the delayed union or nonunion was 11 months. Sample tissues were obtained from the fracture end and its surrounding zone, including fracture ends, medullary cavity contents and the surface scar of bone. Immunohistochemical staining and real time PCR were used to detect the expression of bone morphogenetic protein-2 and decorin of these different tissues.
RESULTS AND CONCLUSION: The expression of bone morphogenetic protein-2 of the surface scar tissues was higher than that of the fracture ends and medullary cavity contents, and the difference was significant (P < 0.05); the expression of decorin of the fracture ends was higher than that of the medullary cavity contents and surface scar tissues, and the difference was significant (P < 0.05). Visibly, tissues' lower capabilities of anti-fibrosis and osteogenesis of the nonunion district was related to the asynchronous highly expression of bone morphogenetic protein-2 and decorin. Therefore, the joint injection of bone morphogenetic protein, such as bone morphogenetic protein-2 and decorin into the bone nonunion district can not only promote bone induction capabilities, but also can enhance the conversion of obsolete scar, and make the treatment effect of nonunion be better.

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