Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (45): 8471-8475.doi: 10.3969/j.issn.2095-4344.2012.45.020

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Bone marrow mesenchymal stem cells transplantation promotes the motor function recovery after spinal cord injury

Liu Sheng-liang1, Fan Ye-wen2, Li Yan2, Zhang Hong-nan2, Xue Meng-meng2, Chen Ming-wei3, Kong Jian3, Liu Xin3, Cui Kai-yu1, Peng Yan1, Wang Duo1, Liu Jing-jing1, Ge Xiao-ping3, Wang Yong4, Wu Shu-liang3   

  1. 1Affiliated Hospital of Harbin Medical University, Harbin 150081, Heilongjiang Province, China; 2Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang Province, China; 3Department of Anatomy, Harbin Medical University, Harbin 150081, Heilongjiang Province, China; 4First Department of Orthopedics, the 211 Hospital of Chinese PLA, Harbin 150080, Heilongjiang Province, China
  • Received:2012-03-15 Revised:2012-05-12 Online:2012-11-04 Published:2012-11-04
  • Contact: Wu Shu-liang, M.D., Professor, Department of Anatomy, Harbin Medical University, Harbin 150081, Heilongjiang Province, China wusl66@hotmail.com Wang Yong, Attending physician, First Department of Orthopedics, the 211 Hospital of Chinese PLA, Harbin 150080, Heilongjiang Province, China PLA211doctor@126.com
  • About author:Liu Sheng-liang, Affiliated Hospital of Harbin Medical University, Harbin 150081, Heilongjiang Province, China Guojili8855@sina.com

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Abstract:

BACKGROUND: The studies have confirmed that the bone marrow mesenchymal stem cells transplantation can be induced to differentiate into neural cells, reconstruct neuronal circuits, and promote axonal regeneration so as to recover of spinal cord function.
OBJECTIVE: To explore the repair effect of bone marrow mesenchymal stem cells on spinal cord injury.
METHODS: Forty C57BL/6 mice were divided into four groups, mice in the sham-operation group did not combat the spinal cord, and mice in the other three groups were used to establish the spinal cord injury model by weight impact method. At 7 days after injury, bone marrow mesenchymal stem cell suspension was injected in treatment group via orbital venous plexus by microinjector; mice in the control group were injected with DEME culture medium in the same dose; mice in the model group were not treated. The extent of spinal cord injury was detected by hematoxylin-eosin staining. Nerve cells differentiated from bone marrow mesenchymal stem cells were identified by immunocytochemistry staining. The growth state of transplanted cells was observed by fluorescent microscope. Motor functional recovery was evaluated by modified Tarlov score.
RESULTS AND CONCLUSION: After the bone marrow mesenchymal stem cells were induced to differentiate for 7 days, positive expression of nuclear factor and glial fibrillary acidic protein could be seen in the cells. Both lower extremities of the mice in the model group were paralyzed, and the mice in the sham-operation group had normal action (P < 0.01). At 2 weeks after transplantation, the symptoms of motor functional deficits in the treatment group was gradually restored, and the recovery in the sham-operation group was not obvious (P < 0.05); at 4 weeks after transplantation, there was no significant difference of the Tarlov score between treatment group and sham-operation group (P > 0. 05). Bone marrow mesenchymal stem cells transplantation can promote the motor functional recovery after spinal cord injury.

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