Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (41): 7621-7624.doi: 10.3969/j.issn.2095-4344.2012.41.005

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Isolation and preparation of Flk-1+ mesenchymal stem cells from mice and its biological characteristics

Zhu Xi-shan, Jiang Ni, Song Yu-guang, Ren Jun   

  1. Department of Medical Oncology, Beijing Shijitan Hospital of Capital Medical University (Ninth Clinical Medical College of Peking University), Beijing 100038, China
  • Received:2012-03-05 Revised:2012-06-23 Online:2012-10-07 Published:2012-10-07
  • About author:Zhu Xi-shan☆, Doctor, Department of Medical Oncology, Beijing Shijitan Hospital of Capital Medical University (Ninth Clinical Medical College of Peking University), Beijing 100038, China zhuxishan@tsinghua.org.cn

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Abstract:

BACKGROUND: The bone marrow mesenchymal stem cells can be widely used, the three-lineage differentiation capacity and immune induction characteristics make it widely used in regenerative medicine.
OBJECTIVE: To investigate the new method to isolate the Flk-1+ mesenchymal stem cells from mouse bone marrow, and to detect the biological characteristics of Flk-1+ mesenchymal stem cells.
METHODS: The mononuclear cells were isolated from the bone marrow and the original mesenchymal stem cells were in vitro cultured and amplified. The cell cycle, phenotype and multilineage differentiation capacity were detected.
RESULTS AND CONCLUSION: The original mesenchymal stem cells derived from mice bone marrow were fibrolbast like, and most of them stayed in G0/G1 phase. Cell phenotype test showed they could highly express Flk1, CD13, CD29, CD44; the bone marrow-derived Flk-1+ mesenchymal stem cells under light microscope showed a fibroblast-like or polygonal adherent growth, and under certain circumstances, they exhibited osteogenic, adipogenic and chondrogenic differentiation. Bone marrow-derived Flk-1+ mesenchymal stem cells have multilineage differentiation potential.

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