Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (32): 6032-6036.doi: 10.3969/j.issn.2095-4344.2012.32.027

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Yougui Wan combined with basic fibroblast growth factors induce the differentiation of rat bone marrow stromal cells into neuron-like cells

Yang Ling1, Zhang Zuo-tao1, Zhang Li-hua2, Lu Ying1   

  1. 1Department of Human Anatomy, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China;
    2Department of Human Anatomy and Embryology, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China
  • Received:2012-05-02 Revised:2012-06-07 Online:2012-08-05 Published:2012-08-05
  • Contact: Zhang Li-hua, Professor, Department of Human Anatomy and Embryology, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China 848774944qq.com
  • About author:Yang Ling★, Master, Department of Human Anatomy, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China 89688173.qq.com.

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Abstract:

BACKGROUND: Previous studies have demonstrated that Chinese medicine for tonifying kidney and benefiting essence can induce bone marrow stromal cells to differentiate into neuron-like cells.
OBJECTIVE: To investigate the differentiation of bone marrow stromal cells into neuron-like cells under the induction of Yougui Wan combined with basic fibroblast growth factor.
METHODS: Bone marrow stromal cells were isolated by attachment and density centrifugation. Passage 3 bone marrow stromal cells were induced to differentiate into neuron-like cells by Yougui Wan combined with basic fibroblast growth factor. Bone marrow stromal cells were divided into three groups: (1) basic fibroblast growth factor group was induced by basic fibroblast growth factor; (2) Yougui Wan+basic fibroblast growth factor group was induced by Yougui Wan and basic fibroblast growth factor; and (3) control group received no administration.
RESULTS AND CONCLUSION: After 7 days of induction, some cells showed typical bipolar and multipolar neuronal morphology. Immunohistochemical staining results showed that neuron specific endolase, nestin and glial fibrillary acidic protein expressions in the Yougui Wan + basic fibroblast growth factor group were significantly greater than in the basic fibroblast growth factor group and control group (P < 0.05). These findings suggest that Yougui Wan combined with basic fibroblast growth factor can induce bone marrow stromal cells to differentiate into neuron-like cells.

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