Abstract:

BACKGROUND: Synovial sarcomas are highly malignant tumor, but there is no effective treatment method. The tumor stem cell theory provides a new approach for the treatment of synovial sarcomas.
OBJECTIVE: To explore whether stem-like cells exist in synovial sarcoma SW982 cell lines.
METHODS: We cultured SW982 cells in a low concentration of serum medium, and the cells which were cultured in the lowest concentration of serum medium were selected for the drug-resistant experiment. The inhibition rate of tumor cells cultured under different concentrations of adriamycin (1, 10, 20, 30, 40, 50, 60 mg/L) were tested by MTT assay. We performed tumorigenicity experiments in the nude mice with the drug-resistant and non-drug-resistant cells respectively.
RESULTS AND CONCLUSION: Drug-resistant capacity of SW982 cells was improved after cultured with low concentration of serum. In the tumorigenicity experiments, there were two nude mice in which a tumor formed after injected with drug-resistant cells; and tumors did not form in the others. There are high tumorigenicity tumor stem-like cells in the SW982 cell lines. We can collect high tumorigenicity synovial sarcoma cell line effectively with high concentration of adriamycin, and enrich the synovial sarcoma stem cells, but the cells need further purification.