Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (32): 5897-5896.doi: 10.3969/j.issn.2095-4344.2012.32.002

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Expression of embryonic stem cell-related transcription factors in human umbilical cord mesenchymal stem cells

Han Zhi-bo1, 2, Chi Ying1, Yang Shao-guang1, Wang You-wei1, 3, Yang Zhou-xin1, Ji Yue-ru1, Yang Ping1, 2, Han Zhong-chao1, 2, 3   

  1. 1State Key Laboratory of Experimental Hematology, Blood Disease Hospital, Hematology Institute, Chinese Academy of Medical Sciences, Tianjin 300020, China;
    2TEDA Life Science and Technology Research Center, Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin 300457, China;
    3National Engineering Research Center of Cell Products, Tianjin 300457, China
  • Received:2012-03-05 Revised:2012-04-16 Online:2012-08-05 Published:2012-08-05
  • Contact: Han Zhong-chao, M.D., Professor, State Key Laboratory of Experimental Hematology, Blood Disease Hospital, Hematology Institute, Chinese Academy of Medical Sciences, Tianjin 300020, China; TEDA Life Science and Technology Research Center, Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin 300457, China; National Engineering Research Center of Cell Products, Tianjin 300457, China hanzhongchao@ hotmail.com
  • About author:Han Zhi-bo, Assistant professor, State Key Laboratory of Experimental Hematology, Blood Disease Hospital, Hematology Institute, Chinese Academy of Medical Sciences, Tianjin 300020, China; TEDA Life Science and Technology Research Center, Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin 300457, China zhibohan@163.com

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Abstract:

BACKGROUND: By regulating gene transcription in embryonic stem cells, Nanog, Oct4 and Sox2 play a key role in the regulation of pluripotency and self-renewal. Expression of embryonic stem cell-associated transcription factors in umbilical cord mesenchymal stem cells has not been fully clarified.
OBJECTIVE: To investigate the expression of embryonic stem cell-associated transcription factor-Nanog, Oct4 and Sox2 in umbilical cord mesenchymal stem cells.
METHODS: Umbilical cord mesenchymal stem cells were cultured with collagenase and trypsinase digestion. Human embryonic stem cells were cultured in the mTeSRTM1 system in the absence of trophoderm. The difference in Nanog, Oct4 and Sox2 mRNA expression was compared between umbilical cord mesenchymal stem cells and embryonic stem cells by quantitative PCR. Nanog, Oct4 and Sox2 expression in umbilical cord mesenchymal stem cells and embryonic stem cells was determined by immunofluorescent staining.
RESULTS AND CONCLUSION: Nanog, Oct4 and Sox2 were expressed in human umbilical cord mesenchymal stem cells. However, Oct4, in particular Oct4B, was mainly located in the cytoplasm of human umbilical cord mesenchymal stem cells. Nanog, Oct4 and Sox2 expression was significantly lower in the umbilical cord mesenchymal stem cells than in the embryonic stem cells, and the mRNA expression of Nanog, Oct4 and Sox2 in the umbilical cord mesenchymal stem cells was 20%, 0.3% and 10% of that in the embryonic stem cells. This study revealed the different expression profiles of Nanog, Oct4 and Sox2 in umbilical cord mesenchymal stem cells and embryonic stem cells. It is beneficial to optimizing the methods of reprogramming umbilical cord mesenchymal stem cells and analyzing the function of embryonic stem cell-associated transcription factors in adult stem cells.

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