Abstract:

BACKGROUND: Preliminary experiments have confirmed that mixture of silk fibroin and chitosan in a proper ratio can make up their shortcomings to show the good physical and chemical properties and biological characteristics.
OBJECTIVE: To observe the growth of bone marrow mesenchymal stem cells (BMSCs) on silk fibroin/chitsan (SF/CS) composite three-dimensional scaffolds.
METHODS: Rabbit BMSCs after induction were seeded on SF/CS scaffolds. Cell adhesion rate was detected, and cell growth was observed under inverted microscope and scanning electron microscopy.
RESULTS AND CONCLUSION: The cell adhesion rate was increased as time went on. Inverted microscope: BMSCs on the scaffolds could not be seen clearly, the number of cells around the scaffolds was increased as time went on, and the cells extended into the scaffolds. Scanning electron microscopy: the cells grew actively and had normal multiple fissions, granular and filamentous substrates could be seen around the cells, and cell microfilaments were closely connected with the scaffolds; the cells could not only show the attached growth on surfaces of the scaffolds, but could also extend into the scaffolds. It is indicated that the SF/CS composite scaffolds have a good cell biocompatibility.