Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (18): 3285-3289.doi: 10.3969/j.issn.1673-8225.2012.18.013

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Heterogeneous islet transplantation via hepatic artery versus portal vein for typeⅠdiabetes in rhesus monkeys   

Ye Bin, Liao Jian, Nie Wei, Liang Qi, Dong Qiong, Ma Xiao-qian, Wang Wei, Liu Sheng   

  1. Department of Radiology, Third Xiangya Hospital of Central South University, Changsha  410013, Hunan Province, China
  • Received:2011-12-14 Revised:2011-12-23 Online:2012-04-29 Published:2012-04-29
  • Contact: Liu Sheng, Doctor, Professor, Chief physician, Master’s supervisor, Department of Radiology, Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China
  • About author:Ye Bin★, Master, Associate chief physician, Department of Radiology, Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China yebin9992003@163.com

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Abstract:

BACKGROUND: Islet transplantation via portal vein and hepatic artery to treat diabetes are the main two ways. The portal vein way has more complications; the hepatic artery transplantation has less trauma and complications.
OBJECTIVE: To compare the efficiency of heterogeneous islet transplantation via hepatic artery and portal vein in the treatment of diabetes rhesus monkeys.
METHODS: Eighteen diabetes rhesus monkey models were divided into three groups: hepatic artery group (n=6), portal vein group (n=9) and control group (n=3). In hepatic artery group, the handled new pig islets (50 000 islet equivalents/kg) were implanted in the models through the hepatic artery catheterization by Seidinger technique; in portal vein group, the handled new pig islets (50 000 islet equivalents/kg) were implanted in the models through the portal venipuncture by ultrasonic guidance; in the control group, two models were preformed with saline injection through the portal venipuncture and one model was performed with saline injection in the same dose with the hepatic artery group via hepatic artery. All groups received the same immunosuppressive regimen. 
RESULTS AND CONCLUSION: The dosage of exogenous insulin administrated in hepatic artery group and portal vein group were decreased to 59.53% and 48.39% respectively. There were no significant differences between hepatic artery group and portal vein group in decreasing dosage of exogenous insulin administration (P > 0.05). Both groups had low level of rhesus serum C peptide, whereas the level of pig serum C petide was increased gradually. There was no significant difference in pig serum C peptide lever between two groups (P > 0.05). However, 4/9 diabetes rhesus monkeys in the portal vein group were dead due to different complications after transplantation, only 1 diabetes rhesus monkey was dead in hepatic artery group due to the puncture point bleeding. The procedure of transplantation via hepatic artery is safer, easier and with fewer complications compared with transplantation via portal vein. The xeno islet grafts can survive for a long time and maintain functional in both approaches.

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