Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (18): 3290-3294.doi: 10.3969/j.issn.1673-8225.2012.18.014

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B7-2/CD28 expression in renal allograft recipients when acute rejection happens  

Shi Li-hua1, 2, Li Ying2, Li Hui2, Sun Guang1   

  1. 1Department of Urology, the Second Hospital of Tianjin Medical University, Tianjin  300000, China; 2Department of Renal Disease, the Affiliated Hospital of Medical College of Chinese People’s Armed Police Force, Tianjin  300162, China
  • Received:2012-01-09 Revised:2012-03-09 Online:2012-04-29 Published:2012-04-29
  • Contact: Li Hui, Doctor, Chief physician, Department of Renal Disease, the Affiliated Hospital of Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China LIHUI2009WUJING@163.com
  • About author:Shi Li-hua☆, Studying for doctorate, Associate chief physician, Department of Urology, the Second Hospital of Tianjin Medical University, Tianjin 300162, China; Department of Renal Disease, the Affiliated Hospital of Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China shilihua1972@163.com
  • Supported by:

     Project of Armed Police Headquarters, No.WKH2006-17*

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Abstract:

BACKGROUND: The results of previous studies on animals indicate that the expression of co-stimulatory molecules in allograft recipients are relate to acute rejection of organs.
OBJECTIVE: To observe the expression of B7-2/CD28 in peripheral blood samples and allograft renal biopsies of allograft recipients when acute rejection happened.
METHODS: The peripheral blood samples of 53 cases were collected on 1 day pre-operative and 1, 3, 7, 14, 21 and 28 days postoperative, and were also collected during episodes of acute rejection and 1 week after treatment of acute rejection. The expression of B7-2/CD28 peripheral blood lymphocytes was examined by flow cyctometry. Allograft receptor’s renal biopsies were taken on pre-transplantation (at the cold ischaemia) and 7 days, 1 and 6 months and 1 year postoperative. Immuohistochemical method was used to detect the expression of B7-2/CD28.
RESULTS AND CONCLUSION: In the post-transplantation period, the number of CD28+, CD4+/CD28+, CD8+/CD28+ cells was decreased significantly within 1 and 3 days (P < 0.05), and then returned to pre-transplant levels by 1 to 2 weeks; increased expression of B7-2 cells was observed in the renal tissue without acute rejection at 7 days followed renal transplantation (P < 0.05), and then returned to the level of preoperative tissue after 1 month (P > 0.05). When acute rejection happened, the ratios of CD28+, CD4+/CD28+ and CD8+/CD28+ cells of peripheral blood samples and the expression of B7-2 in renal biopsies samples were significantly increased (P < 0.05) and returned to normal immediately after anti-rejection therapy for 1 week. The results show that the increased expression of co-stimulatory molecule B7-2/CD28 in allograft renal biopsies and peripheral blood samples of allograft recipients were closely relate to acute rejection after clinical renal transplantation.

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