Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (14): 2648-2651.doi: 10.3969/j.issn.1673-8225.2012.14.037

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Detection of interleukin 2, interleukin 10, transforming growth factor β and CD4+CD25+ regulatory T cells in peripheral blood of patients with acute lymphoblastic leukemia 

Wu Cui-Ping1, Qin Xi1, Wu Cui-yun2, Zhu Hong1, Zhou Hai-yan1   

  1. 1The Affiliated Hospital of Hainan Medical College, Haikou  570102, Hainan Province, China; 2People’s Hospital of Haikou, Haikou  570208, Hainan Province, China
  • Received:2011-08-08 Revised:2011-10-30 Online:2012-04-01 Published:2012-04-01
  • Contact: Qin Xi, Professor, the Affiliated Hospital of Hainan Medical College, Haikou 570102, Hainan Province, China qinxi99@21cn.com
  • About author:Wu Cui-Ping, Associate chief technician, the Affiliated Hospital of Hainan Medical College, Haikou 570102, Hainan Province, China wucuiping2007@163.com
  • Supported by:
    Reaearch Project Topics of HealthyDepartment of Hainan Province, No. Qiongwei2008-55*
     

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Abstract:

BACKGROUND: At present, the cause of acute lymphoblastic leukemia (ALL) has not yet entirely understood, CD4+CD25+ regulatory T cells (Treg) may have certain regulatory effect on immune response in pathogenic process of ALL.
OBJECTIVE: To study the levels of interleukin 2 (IL-2), interleukin 10 (IL-10), transforming growth factor (TGF-β) of CD4+CD25+ regulatory T cells (Treg) in peripheral blood of patients with ALL.
METHODS: The peripheral blood mononuclear cells (PBMC) were isolated with density gradient centrifugation from 22 B cell series ALL (B-ALL) patients, 13 T cell series ALL (T-ALL) patients and 18 normal people. Immunomagnetic bead separation was used to separate CD4+CD25+Treg cells, and the levels of IL-2, IL-10 and TGF-β in the cells culture supernatant were detected by using enzyme-linked immunosorbent assay (ELISA).
RESULTS AND CONCLUSION: The levels of IL-10 and TGF-β in the cells culture supernatant of B-ALL patients and T-ALL patients were significantly higher than those in normal people, while the level of IL-2 was significantly lower than that in normal people (P < 0.05). There was no significant difference between B-ALL and T-ALL patients in the IL-2, IL-10 and TGF-β levels of the cells culture supernatant (P > 0.05). CD4+CD25+Treg cells might interfere the immunologic reaction of antitumor by increasing the IL-10 and TGF-β levels and decreasing the IL-2 level.
 

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