Abstract:

BACKGROUND: Stem cells proliferated and differented to form fibroblasts and well-suited connective tissue, followed by tissue regeneration, which is a gradually increasing research area for treatment of pelvic organ prolapse and stress urinary incontinence.
OBJECTIVE: To investigate the feasibility of periurinary tract infection of autologous adipose-derived stem cells combined with fibroblasts for treatment of stress urinary incontinence.
METHODS: Rats were induced to develop stress urinary transplantation by postpartum vaginal balloon dilation and bilateral ovariectomy. One month later, autologous adipose-derived stem cells and fibroblasts were injected into the region surrounding the urinary tract. Another month later, urinary voiding function was assessed by conscious cystometry. At the same time, urinary tract proximal end tissue was stained by hematoxylin-eosin and elastic fibers were stained to observe morphological change.
RESULTS AND CONCLUSION: Compared with un-treated rats, rats receiving stem cell treatment exhibited increased leak point pressure (P < 0.01) with normal voiding, thickened urinary tract muscular layer (P < 0.01) and increased elastic fiber and smooth muscle content (P < 0.01). These findings suggest that periurinary tract infection of autologous adipose-derived stem cells combined with fibroblasts can be used for treatment of stress urinary incontinence because it can significantly increase abdominal leak point pressure and strengthen urinary tract muscular layer pressure.