Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (14): 2496-2504.doi: 10.3969/j.issn.1673-8225.2012.14.006

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Salidrosides induced rat bone marrow mesenchymal stem cells to differentiate into neuron-like cells in vitro

Zhang Quan-wei1, 2, Zhao Xing-xu1, Zhao Hong-bin2, Zhang Ming2, Yang Yin-shu3, Ge Bao-feng2◇   

  1. 1College of Life Sciences, Gansu Agricultural University, Lanzhou  730070, Gansu Province, China; 2Institute of Bone Injury, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou  730050, Gansu Province, China;
    3 Lanzhou Military Region Center for Disease Control and Prevention of Chinese PLA, Lanzhou  730020, Gansu Province, China
  • Received:2011-09-06 Revised:2011-09-28 Online:2012-04-01 Published:2012-04-01
  • Contact: author: Zhao Hong-bin, Doctor, Master’s supervisor, Associate chief technician, Institute of Bone Injury, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu Province, China zhao6703@yahoo.com.cn
  • About author:Zhang Quan-wei★, Studying for master’s degree, College of Life Sciences, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China; Institute of Bone Injury, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu Province, China zqwfree826@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 81073156*

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Abstract:

BACKGROUND: Preliminary experiments have demonstrated that salidroside (SD) can induce bone marrow mesenchymal stem cells (BMSCs) to differentiate into neural-like cells. However, little is known about how SD affects BMSCs differentiation into neural-like cells.
OBJECTIVE: To investigate the molecular mechanism by which SD induces BMSCs to differentiate into neural-like cells.
METHODS: Passage 2 Wistar rat BMSCs were divided into a retinoic acid (RA) group, SD group, and a control group. Cells were observed at different time points.
RESULTS AND CONCLUSION: The expression levels of neural stem cell marker nestin, neural cell differentiation-related to rabbit anti-microtubule protein and microtubule-associated protein 2 (MAP2) in the RA and SD groups were significantly higher than those in the control group at each time point (P < 0.01). The expression level of glial fibrillary acidic protein (GFAP) in the RA group was significantly higher than that in the SD group (P < 0.01). Real-time PCR results suggested that there were neuron-specific enolase (NSE) and MAP2 mRNA expressions in the RA and SD groups at each time point. The expression level depended but not absolutely on induction time. The high abundance of GFAP mRNA in the RA and SD groups appeared in the early stage of induction. NSE protein expression in the RA and SD groups was significantly increased with prolongation of induction time and rabbit anti-microtubule protein appeared in the early stage of induction. These findings suggest that SD, similar to RA, can promote BMSCs to differentiate toward neural cells, but it exhibits better effects on differentiation towards neural-like cells than RA.
 

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