Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (6): 959-962.doi: 10.3969/j.issn.1673-8225.2011.06.003

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Ultrastructural characteristics of adult bone marrow mesenchymal stem cells labeled with green fluorescent protein

Ma Li-hua, Zhao Yi, Cai Xue-min, Deng Yong-li, Pan Xing-hua   

  1. Research Center of Stem Cell, Tissue and Organ Engineering, Kunming General Hospital of Chinese PLA, Kunming  650032, Yunnan Province, China
  • Received:2010-09-20 Revised:2010-10-29 Online:2011-02-05 Published:2011-02-05
  • Contact: Pan Xing-hua, Professor, Master’s supervisor, Research Center of Stem Cell, Tissue and Organ Engineering, Kunming General Hospital of Chinese PLA, Kunming 650032, Yunnan Province, China
  • About author:Ma Li-hua, Intern Researcher, Research Center of Stem Cell, Tissue and Organ Engineering, Kunming General Hospital of Chinese PLA, Kunming 650032, Yunnan Province, China Yangrhua@msn.com
  • Supported by:

    the Natural Science Foundation of Yunnan Province, No. 2003C00132*

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Abstract:

BACKGROUND: Studies have suggested that no significant changes were determined in immunological phenotype, cell cycle and differentiation potential of bone marrow mesenchymal stem cells (BMSCs) labeled with green fluorescent protein (GFP).
OBJECTIVE: To further study effects of GFP on ultrastructure of BMSCs.
METHODS: BMSCs were isolated from adult marrow and cultured in vitro. BMSCs were stained in immunocytochemistry with antibody of anti-CD34 and anti-CD105 to identify. The third generation of BMSCs were labeled with GFP. Ultrathin sections of BMSCs labeled with GFP were observed for ultrastructure under the transmission electron microscope, and normal BMSCs without GFP served as controls.
RESULTS AND CONCLUSION: BMSCs expressed CD105 and not CD34. They labeled with GFP had green fluorescence in the cytoplasm. The fluorescent strength became weak gradually in the cytoplasm away from nuclei. BMSCs labeled GFP had many rough endoplasm reticulum and Golgi body, less mitochondrion than normal BMSCs. In addition, lipid droplet and “vacuole”-like structure became more. Results verified that effects of GFP on BMSCs were limited, and GFP was an ideal tracing reagent.

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