Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (6): 1044-1049.doi: 10.3969/j.issn.1673-8225.2011.06.021

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Effect of Buyang Huanwu decoction on number and function of endothelial progenitor cells in the peripheral blood 

Liu Kun1, 2, Li Kun3, Dong Guo-hua2, Shen Yi2, Wang Chang-tian2, Wu Hai-wei2, Jing Hua2   

  1. 1Department of Cardiovascular Surgery, Affiliated Hospital of Nantong University, Nantong  226001, Jiangsu Province, China
    2General Hospital of Nanjing Military Area Command of Chinese PLA, Nanjing University Medical School, Nanjing 210093, Jiangsu Province, China
    3Department of Vascular Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210093 Jiangsu Province, China
  • Received:2010-08-11 Revised:2010-10-16 Online:2011-02-05 Published:2011-02-05
  • About author:Liu Kun☆, Studying for doctorate, Associate chief physician, Department of Cardiovascular Surgery, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China; General Hospital of Nanjing Military Area Command of Chinese PLA, Nanjing University Medical School, Nanjing 210093, Jiangsu Province, China jsntliukun@sina.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30972969*

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Abstract:

BACKGROUND: Modern medicine studies have confirmed that endothelial progenitor cells (EPCs) and Buyang Huanwu Decoction (BHD) have similar functions for treatment of ischemia disease. Is there a correlation between them?
OBJECTIVE: To explore the effects of BHD on the number and function of EPCs in peripheral blood.
METHODS: EPCs were isolated from rabbit peripheral blood by density gradient centrifugation. Cultured EPCs were identified as Dil-LDL uptaking and FITC-lectin binding and further demonstrated using surface antigen immunohistochemistry. The attached cells were randomized into four groups. After being cultured in basal medium and EBM-2 medium containing different concentrations of BHD extract (20, 50, 100 g/L) for 72 hours, the cell morphology was observed. Meanwhile, the proliferation, migration, and adhesion of EPCs were assayed with MTT, Transwell chamber and adhesion function test, respectively. In addition, nitric oxide content and vascular endothelial growth factor (VEGF) expression were assessed. 
RESULTS AND CONCLUSION: Various mass concentrations of BHD could increase the number of EPCs, elevated proliferation, adherence, migration and nitric oxide secretion capacity of EPCs (P < 0.05). The effect was most significant at 50 g/L (P < 0.01). However, the effect was weak on VEGF expression. Results indicated that BHD could significantly increase the number of EPCs, and enhance the proliferation, migration, and adhesive capacity of EPCs. Meanwhile, BHD can induce late EPCs differentiation.

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