Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (6): 1015-1019.doi: 10.3969/j.issn.1673-8225.2011.06.015

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Expression of stem cell-associated genes in colorectal cancer and SW620 cell line

Li Qiu-xian1, Huang Ying-feng2, Wei Ya-ming3, Wang Xiao-hua1, Luo Hong-tu3, Yang Shao-yu3, Xu Yan-li3, Huang Yu-yi1, Ye Heng1   

  1. 1Biochemistry Teaching and Research Section, Guangzhou Medical College, Guangzhou  510180, Guangdong Province, China
    2Department of General Surgery, Guangzhou Municipal First People’s Hospital, Guangzhou  510180, Guangdong Province, China
    3Guangzhou Institute of Clinical Medicine, Guangzhou Municipal First People’s Hospital, Guangzhou 510180, Guangdong Province, China
  • Received:2010-08-19 Revised:2010-11-20 Online:2011-02-05 Published:2011-02-05
  • Contact: Huang Ying-feng, Master, Chief physician, Department of General Surgery, Guangzhou Municipal First People’s Hospital, Guangzhou 510180, Guangdong Province, China huangyinfeng@yahoo.com.cn Wei Ya-ming, Doctor, Researcher, Guangzhou Institute of Clinical Medicine, Guangzhou Municipal First People’s Hospital, Guangzhou 510180, Guangdong Province, China weiyaming@163.com
  • About author:Li Qiu-xian★, Studying for master’s degree, Biochemistry Teaching and Research Section, Guangzhou Medical College, Guangzhou 510180, Guangdong Province, China 178248744@qq.com
  • Supported by:

    the Medical Scientific Research Foundation of Guangdong Province, No. A2008517*; the Science and Technology Project of Guangzhou City, No. 2008J1-C171*

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Abstract:

BACKGROUND: Tumor cells always show some stem cell markers. The expression of these markers will help us to understand the mechanism of tumorgenesis and to perfect the theory of tumor stem cells.
OBJECTIVE: To investigate the expression of stem cell-related molecules markers CD133, CD166, Oct4, Sox2, C-myc, Klf4 and Bmi-1 in colorectal cancer tissue, the difference compared with the expression of adjacent tissues, as well as the difference in expression of above-described genes in sorted CD133+ SW620 cell line.
METHODS: RNA was extracted from cancer tissues of 29 colon cancer tissue specimens. The expression of CD133, CD166, Oct4, Sox2, C-myc, Klf4 and Bmi-1 was detected by RT-PCR in colon cancer tissues and adjacent tissues. CD133+ SW620 cells were sorted, and RNA was extracted from CD133+ cells and CD133- cells. The different expressions of above genes were detected using RT-PCR.
RESULTS AND CONCLUSION: All samples were proved to be poorly/moderately differentiated adenocarcinoma or myxoadenocarcinoma in 29 cases. The relative quantity expressions of the stem cell-related molecules markers in the cancer tissue were higher than adjacent tissues (P < 0.05). RT-PCR indicated that stem cell associated genes expressions (CD133, CD166, Oct4, Sox2, C-myc, Klf4, Bmi-1) in CD133+ sorted cells were higher than that in CD133- ones. These suggested that CD133, CD166, Oct4, Sox2, C-myc, Klf4 and Bmi-1 were used in colorectal cancer diagnosis.

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