Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (6): 1005-1008.doi: 10.3969/j.issn.1673-8225.2011.06.013

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Biological characteristics and anti-apoptotic cytokine secretion levels of human placenta mesenchymal stem cells

Zhu Shao-fang1, He Yuan-li1, Lu Guo-hui2, Fu Xia-fei1   

  1. 1Department of Obstetrics and Gynecology, 2Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou  510282, Guangdong Province, China
  • Received:2010-08-21 Revised:2010-10-13 Online:2011-02-05 Published:2011-02-05
  • Contact: He Yuan-li, Professor, Doctoral supervisor, Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China
  • About author:Zhu Shao-fang☆, Studying for doctorate, Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China zhushaofang526@126.com

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Abstract:

BACKGROUND: The secondary injury to patients by extraction of bone marrow mesenchymal stem cells also has the possibility of virus and bacterial contamination, and the ability of amplification and differentiation was not high. Therefore, this study extracted mesenchymal stem cells from placenta to understand the condition of secreting anti-apoptosis cytokine, and to explore the possibility of replacing bone marrow mesenchymal stem cells.
OBJECTIVE: To isolate, culture in vitro, amplify human placenta derived mesenchymal stem cells, and to study the biological character and secretion levels of placenta derived mesenchymal stem cells.
METHODS: After isolating mesenchymal stem cells from the placenta, cell morphology was observed under microscope. Cell cycle, apoptotic cell death and the surface labeling expression were evaluated using flow cytometry to study their proliferation and growth characteristics. The cytokines, including vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF) and insulin-like growth factor-1 (IGF-1), were detected using enzyme-linked immunosorbent assay.
RESULTS AND CONCLUSION: Human placenta mesenchymal stem cells were fibroblast-like, with increased nuclear cytoplasm ratio under in vitro culture. The proliferation ratio was increased, while apoptosis decreased. Human placenta mesenchymal stem cells secreted anti-apoptotic cytokines, such as VEGF, IGF-1, and HGF.

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